Structure of PDB 7z34 Chain m

Receptor sequence

>7z34m (length=730) Species: 559292 (Saccharomyces cerevisiae S288C)

KLPAEFITRPHPSKDHGKETCTAYIHPNVLSSLEINPGSFCTVGKIGENG
ILVIARAGDEEVHPVNVITLSTTIRSVGNLILGDRLELKKAQVQPPYATK
VTVGSLQGYNILECMEEKVIQKLLDDSGVIMPGMIFQNLKTKAGDESIDV
VITDASNLFYLSPPFIFRKGSTHITFSKETQANRKYNLPEPLSYAAVGGL
DKEIESLKSAIEIPLHQPTLFSSFGVSPPRGILLHGPPGTGKTMLLRVVA
NTSNAHVLTINGPSIVSKYLGETEAALRDIFNEARKYQPSIIFIDEIDSI
APNRANDDSGEVESRVVATLLTLMDGMGAAGKVVVIAATNRPNSVDPALR
RPGRFDQEVEIGIPDVDARFDILTKQFSRMSSDRHVLDSEAIKYIASKTH
GYVGADLTALCRESVMKTIQRGLGTDANIDKFSLKVTLKDVESAMVDIRP
SAMREIFLEMPKVYWSDIGGQEELKTKMKEMIQLPLEASETFARLGISAP
KGVLLYGPPGCSKTLTAKALATESGINFLAVKGPEIFNKYVGESERAIRE
IFRKARSAAPSIIFFDEIDALSPDRDGSSTSAANHVLTSLLNEIDGVEEL
KGVVIVAATNRPDEIDAALLRPGRLDRHIYVGPPDVNARLEILKKCTKKF
NTEESGVDLHELADRTEGYSGAEVVLLCQEAGLAAIMEDLDVAKVELRHF
EKAFKGIARGITPEMLSYYEEFALRSGSSS

3D structure

• PDB: 7z34 Visualizing maturation factor extraction from the nascent ribosome by the AAA-ATPase Drg1.
• Chain: m
• Resolution: 3.8 Å

Enzymatic activity

• Enzyme Commision number: 3.6.4.10: non-chaperonin molecular chaperone ATPase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	m	K318 K589 V591 S629	K268 K539 V541 S579
BS02	AGS	m	R671 R674	R621 R624
BS03	AGS	m	V247 P288 G289 K292 M294 E346 I422 A455 T458	V197 P238 G239 K242 M244 E296 I372 A405 T408
BS04	AGS	m	D517 G560 C561 S562 K563 L565 I692 K695 G721 A722 V725	D467 G510 C511 S512 K513 L515 I642 K645 G671 A672 V675

Gene Ontology

Molecular Function

• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0016787 hydrolase activity
• GO:0016887 ATP hydrolysis activity

Biological Process

• GO:0009410 response to xenobiotic stimulus
• GO:0034214 protein hexamerization
• GO:0042254 ribosome biogenesis
• GO:0042273 ribosomal large subunit biogenesis

Cellular Component

• GO:0005737 cytoplasm
• GO:0030687 preribosome, large subunit precursor

External links

• PDB: RCSB:7z34, PDBe:7z34, PDBj:7z34
• PDBsum: 7z34
• PubMed: 36097293
• UniProt: P32794|AFG2_YEAST ATPase family gene 2 protein (Gene Name=AFG2)