Structure of PDB 6wdm Chain m

Receptor sequence

>6wdmm (length=136) Species: 562 (Escherichia coli)

MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTARQIEAAR
RAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGK
VLYEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM

3D structure

• PDB: 6wdm Cryo-EM of elongating ribosome with EF-Tu•GTP elucidates tRNA proofreading.
• Chain: m
• Resolution: 3.6 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	m	K5 R6 F9 M12 H13 K14 G15 R16 Q22 G23 R40 R44 Q45 R55 W64 R66 F68 D70 T74 E75 K76 R81 M82 G83 K84 G85 K86 G87 K100 L119 A122 K123 P125 K127	K5 R6 F9 M12 H13 K14 G15 R16 Q22 G23 R40 R44 Q45 R55 W64 R66 F68 D70 T74 E75 K76 R81 M82 G83 K84 G85 K86 G87 K100 L119 A122 K123 P125 K127
BS02	rna	m	G19 R38	G19 R38
BS03	rna	m	R55 R59	R55 R59

Gene Ontology

Molecular Function

• GO:0000049 tRNA binding
• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:6wdm, PDBe:6wdm, PDBj:6wdm
• PDBsum: 6wdm
• PubMed: 32612237
• UniProt: P0ADY7|RL16_ECOLI Large ribosomal subunit protein uL16 (Gene Name=rplP)