Structure of PDB 6fyy Chain m

Receptor sequence

>6fyym (length=147) Species: 4932 (Saccharomyces cerevisiae)

INICRDNHDPFYRYKMPPIQAKVEGRGNGIKTAVLNVADISHALNRPAPY
IVKYFGFELGAQTSISVDKDRYLVNGVHEPAKLQDVLDGFINKFVLCGSC
KNPETEIIITKDNDLVRDCKACGKRTPMDLRHKLSSFILKNPPDSVS

3D structure

• PDB: 6fyy Translational initiation factor eIF5 replaces eIF1 on the 40S ribosomal subunit to promote start-codon recognition.
• Chain: m
• Resolution: 3.02 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	m	E26 R28 G29 N30 G31 K71 D72 R73	E24 R26 G27 N28 G29 K69 D70 R71
BS02	rna	m	K55 F59 E60 L61 G62 A63 Q64 N77 G78 K135 K142	K53 F57 E58 L59 G60 A61 Q62 N75 G76 K133 K140

Gene Ontology

Molecular Function

• GO:0003743 translation initiation factor activity
• GO:0005092 GDP-dissociation inhibitor activity
• GO:0005096 GTPase activator activity
• GO:0005515 protein binding
• GO:0005525 GTP binding
• GO:0008270 zinc ion binding
• GO:0031369 translation initiation factor binding
• GO:0071074 eukaryotic initiation factor eIF2 binding

Biological Process

• GO:0001732 formation of cytoplasmic translation initiation complex
• GO:0006412 translation
• GO:0006413 translational initiation
• GO:0006446 regulation of translational initiation
• GO:0042256 cytosolic ribosome assembly
• GO:0045947 negative regulation of translational initiation

Cellular Component

• GO:0005829 cytosol
• GO:0033290 eukaryotic 48S preinitiation complex
• GO:0043614 multi-eIF complex

External links

• PDB: RCSB:6fyy, PDBe:6fyy, PDBj:6fyy
• PDBsum: 6fyy
• PubMed: 30475211
• UniProt: P38431|IF5_YEAST Eukaryotic translation initiation factor 5 (Gene Name=TIF5)