Structure of PDB 5fci Chain l3

Receptor sequence

>5fcil3 (length=386) Species: 559292 (Saccharomyces cerevisiae S288C)

SHRKYEAPRHGHLGFLPRKRAASIRARVKAFPKDDRSKPVALTSFLGYKA
GMTTIVRDLDRPGSKFHKREVVEAVTVVDTPPVVVVGVVGYVETPRGLRS
LTTVWAEHLSDEVKRRFYKNWYKSKKKAFTKYSAKYAQDGAGIERELARI
KKYASVVRVLVHTQIRKTPLAQKKAHLAEIQLNGGSISEKVDWAREHFEK
TVAVDSVFEQNEMIDAIAVTKGHGFEGVTHRWGTKKLPRKTHRGLRKVAC
IGACHPAHVMWSVARAGQRGYHSRTSINHKIYRVGKGDDEANGATSFDRT
KKTITPMGGFVHYGEIKNDFIMVKGCIPGNRKRIVTLRKSLYTNTSRKAL
EEVSLKWIDTASKFGKGRFQTPAEKHAFMGTLKKDL

3D structure

• PDB: 5fci ?
• Chain: l3
• Resolution: 3.4 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	l3	S2 H3 R4 K5 Y6 E7 A8 R10 H11 H13 L14 G15 F16 L17 P18 R19 K20 R21 I25 R26 R28 K30 A31 M53 T54 T55 R62 G64 A75 Y92 T95 R97 L99 R100 S101 L102 T103 T104 W106 R117 F118 Y119 K120 N121 Y123 K124 S125 K126 K128 A129 F130 K132 Y133 R150 K153 Y154 R159 Q173 K174 K175 L178 E180 T221 K222 G223 H224 G225 F226 G228 R232 K236 K237 R240 K241 T242 H243 R244 G245 L246 R247 K248 A250 C251 I252 G253 A254 C255 H256 P257 A258 H259 V260 W262 S263 R266 A267 Q269 R270 S274 R275 I278 N279 G309 H313 G315 K325 C327 I328 R332 K333 R334 R339 L342 Y343 R348 K349 S363 K364 F365 G366 K367 M380 K384 K385	S1 H2 R3 K4 Y5 E6 A7 R9 H10 H12 L13 G14 F15 L16 P17 R18 K19 R20 I24 R25 R27 K29 A30 M52 T53 T54 R61 G63 A74 Y91 T94 R96 L98 R99 S100 L101 T102 T103 W105 R116 F117 Y118 K119 N120 Y122 K123 S124 K125 K127 A128 F129 K131 Y132 R149 K152 Y153 R158 Q172 K173 K174 L177 E179 T220 K221 G222 H223 G224 F225 G227 R231 K235 K236 R239 K240 T241 H242 R243 G244 L245 R246 K247 A249 C250 I251 G252 A253 C254 H255 P256 A257 H258 V259 W261 S262 R265 A266 Q268 R269 S273 R274 I277 N278 G308 H312 G314 K324 C326 I327 R331 K332 R333 R338 L341 Y342 R347 K348 S362 K363 F364 G365 K366 M379 K383 K384
BS02	OHX	l3	R167 T169 L171	R166 T168 L170
BS03	MG	l3	M53 T77 C327	M52 T76 C326
BS04	MG	l3	A265 R266	A264 R265

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0002181 cytoplasmic translation
• GO:0006364 rRNA processing
• GO:0006412 translation
• GO:0006414 translational elongation
• GO:1990145 maintenance of translational fidelity

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:5fci, PDBe:5fci, PDBj:5fci
• PDBsum: 5fci
• PubMed: 26906928
• UniProt: P14126|RL3_YEAST Large ribosomal subunit protein uL3 (Gene Name=RPL3)