Structure of PDB 8civ Chain l

Receptor sequence

>8civl (length=184) Species: 4932 (Saccharomyces cerevisiae)

GISRDSRHKRSATGAKRAQFRKKRKFELGRQPANTKIGAKRIHSVRTRGG
NKKYRALRIETGNFSWASEGISKKTRIAGVVYHPSNNELVRTNTLTKAAI
VQIDATPFRQWFEAHYGQTLGKAERKWAARAASAKIESSVESQFSAGRLY
ACISSRPGQSGRCDGYILEGEELAFYLRRLTAKK

3D structure

• PDB: 8civ mRNA reading frame maintenance during eukaryotic ribosome translocation
• Chain: l
• Resolution: 2.47 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	l	R92 T93 A162 R164	R91 T92 A146 R148
BS02	rna	l	G2 R5 D6 S7 R8 H9 K10 R11 A13 T14 G15 A16 A19 R22 K23 K24 R25 K26 F27 L29 G30 R31 Q32 P33 A34 R42 I43 H44 S45 R47 T48 R49 G50 G51 N52 K54 R56 L58 R59 N64 S66 G71 I72 S73 K75 S86 N87 T97 K98 A99 K142 R146 S171 R172 G174 Q175 S176 R178 D180	G1 R4 D5 S6 R7 H8 K9 R10 A12 T13 G14 A15 A18 R21 K22 K23 R24 K25 F26 L28 G29 R30 Q31 P32 A33 R41 I42 H43 S44 R46 T47 R48 G49 G50 N51 K53 R55 L57 R58 N63 S65 G70 I71 S72 K74 S85 N86 T96 K97 A98 K126 R130 S155 R156 G158 Q159 S160 R162 D164

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022627 cytosolic small ribosomal subunit
• GO:0030686 90S preribosome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8civ, PDBe:8civ, PDBj:8civ
• PDBsum: 8civ
• PubMed: 38030725
• UniProt: P0CX40|RS8B_YEAST Small ribosomal subunit protein eS8B (Gene Name=RPS8B)