Structure of PDB 6wdf Chain k

Receptor sequence

>6wdfk (length=122) Species: 83333 (Escherichia coli K-12)

MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIKEAIPRG
KVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIF
GPVTRELRSEKFMKIISLAPEV

3D structure

• PDB: 6wdf Cryo-EM of elongating ribosome with EF-Tu•GTP elucidates tRNA proofreading.
• Chain: k
• Resolution: 3.3 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	k	N13 R17 P48 R98 F100 R105 R108 M113 P120 V122	N13 R17 P48 R98 F100 R105 R108 M113 P120 V122
BS02	rna	k	M1 Q3 E4 Q5 T6 M7 I22 K23 L25 G26 S28 H29 R30 R31 Y32 K40 K54 V57 K66 K67 R70 G74 N82	M1 Q3 E4 Q5 T6 M7 I22 K23 L25 G26 S28 H29 R30 R31 Y32 K40 K54 V57 K66 K67 R70 G74 N82

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding
• GO:0019843 rRNA binding
• GO:0070180 large ribosomal subunit rRNA binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0015934 large ribosomal subunit
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:6wdf, PDBe:6wdf, PDBj:6wdf
• PDBsum: 6wdf
• PubMed: 32612237
• UniProt: P0ADY3|RL14_ECOLI Large ribosomal subunit protein uL14 (Gene Name=rplN)