Structure of PDB 8cgd Chain i

Receptor sequence

>8cgdi (length=141) Species: 679895 (Escherichia coli BW25113)

KTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEYTPHVDTG
DYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVI
EIAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI

3D structure

• PDB: 8cgd Structural conservation of antibiotic interaction with ribosomes.
• Chain: i
• Resolution: 1.98 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	i	K2 T3 T5 K7 E9 W15 T24 G26 R27 T30 R37 K39 H47 Y53 T65 K68 K72 Y75 H76 H77 T78 H80 I81 G82 I84 R95 R96 E102 A104 G107 M108 P113 L114 R116 R120 K123 N131 H132 A134 Q135 Q136	K1 T2 T4 K6 E8 W14 T23 G25 R26 T29 R36 K38 H46 Y52 T64 K67 K71 Y74 H75 H76 T77 H79 I80 G81 I83 R94 R95 E101 A103 G106 M107 P112 L113 R115 R119 K122 N130 H131 A133 Q134 Q135

Gene Ontology

Molecular Function

• GO:0003729 mRNA binding
• GO:0003735 structural constituent of ribosome
• GO:0008270 zinc ion binding
• GO:0048027 mRNA 5'-UTR binding
• GO:0070180 large ribosomal subunit rRNA binding

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation
• GO:0017148 negative regulation of translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8cgd, PDBe:8cgd, PDBj:8cgd
• PDBsum: 8cgd
• PubMed: 37550453
• UniProt: P0AA10|RL13_ECOLI Large ribosomal subunit protein uL13 (Gene Name=rplM)