Structure of PDB 8kab Chain h

Receptor sequence

>8kabh (length=430) Species: 246196 (Mycolicibacterium smegmatis MC2 155)

YRQLRLERVVLVGVWTEGSAADAEASLAELAALAETAGSEVLEGLIQRRD
KPDPSTYIGSGKAAELREVVLATGADTVICDGELSPAQLNALEKAVKVKV
IDRTALILDIFAQHATSREGKAQVSLAQMEYMLPRLRGWGESMSRQAGGR
AGGAGGGVGTRGPGETKIETDRRRIRERMAKLRREIRDMKKIRDTQRGSR
RRSEIPSVAIVGYTNAGKSSLLNALTGAGVLVENALFATLEPTTRRGEFE
DGRPFVLTDTVGFVRHLPTQLVEAFRSTLEEVVDADLLIHVVDGSDVNPL
AQINAVRTVINEVVAEYDIAPPPELLVVNKIDAATGVGLAQLRRALPDAV
FVSARTGDGLDKLRSRMGELVESTDATVDVTIPYDRGDLVARVHTDGHVD
ATEHTDAGTRIKARVPAPLAATLREYATFA

3D structure

• PDB: 8kab Cryo-EM structures reveal the molecular mechanism of HflX-mediated erythromycin resistance in mycobacteria.
• Chain: h
• Resolution: 3.3 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	h	Q87 G99 S100 G101 K102 T156 S157 R158 R190 A191 G192 G193 A194 G195 G196 G197 V198 G199 T200 R201 G202 P203 G204 T206 K207 I208 E209 R213 R214 E217 R218 K221 I232 R233 Q236 R240 T284 R285 R286 A431 H434 P458	Q47 G59 S60 G61 K62 T116 S117 R118 R150 A151 G152 G153 A154 G155 G156 G157 V158 G159 T160 R161 G162 P163 G164 T166 K167 I168 E169 R173 R174 E177 R178 K181 I192 R193 Q196 R200 T244 R245 R246 A391 H394 P418
BS02	GNP	h	N255 E273 L276 F277 K370 R395	N215 E233 L236 F237 K330 R355

Gene Ontology

Molecular Function

• GO:0003924 GTPase activity
• GO:0005525 GTP binding
• GO:0043022 ribosome binding
• GO:0046872 metal ion binding

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:8kab, PDBe:8kab, PDBj:8kab
• PDBsum: 8kab
• PubMed: 39029461
• UniProt: A0QVY1