Structure of PDB 8phj Chain f

Receptor sequence

>8phjf (length=177) Species: 562 (Escherichia coli)

AKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAIADKKLLD
NAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFE
RLITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRG
LDITITTTAKSDEEGRALLAAFDFPFR

3D structure

• PDB: 8phj Ribosomal stalk-captured CARF-RelE ribonuclease inhibits translation following CRISPR signaling
• Chain: f
• Resolution: 3.67 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	f	S73 V74 A75 R80	S72 V73 A74 R79
BS02	rna	f	K33 N37 M38 G39 G41 E42 T68 R71 K72 F77 I79 K88 S121 F122 D123 R125 N127 S129 R133 G151 L152 D153	K32 N36 M37 G38 G40 E41 T67 R70 K71 F76 I78 K87 S120 F121 D122 R124 N126 S128 R132 G150 L151 D152
BS03	rna	f	S24 M26 Q27 Q63 K64 L66 K69 T90 R92	S23 M25 Q26 Q62 K63 L65 K68 T89 R91

Gene Ontology

Molecular Function

• GO:0000049 tRNA binding
• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0008097 5S rRNA binding
• GO:0019843 rRNA binding

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8phj, PDBe:8phj, PDBj:8phj
• PDBsum: 8phj
• PubMed: 38033086
• UniProt: P62399|RL5_ECOLI Large ribosomal subunit protein uL5 (Gene Name=rplE)