Structure of PDB 8ifb Chain f

Receptor sequence

>8ifbf (length=177) Species: 562 (Escherichia coli) [Search protein sequence]

AKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAIADKKLLD
NAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFE
RLITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRG
LDITITTTAKSDEEGRALLAAFDFPFR

3D structure

PDB

8ifb Direct visualization of ribosomes in the cell-free system revealed the functional evolution of aminoglycoside.

Chain

Resolution

2.43 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	rna	f	K33 T35 N37 M38 G39 G41 T68 A70 R71 K72 F77 I79 I85 S121 F122 D123 R125 N127 S129 M130 G131 G151 L152 D153 T155 T157	K32 T34 N36 M37 G38 G40 T67 A69 R70 K71 F76 I78 I84 S120 F121 D122 R124 N126 S128 M129 G130 G150 L151 D152 T154 T156
BS02	rna	f	S24 V25 M26 Q27 Q63 K64 L66 K69 K88 T90 R92	S23 V24 M25 Q26 Q62 K63 L65 K68 K87 T89 R91
BS03	rna	f	S73 A75 R80	S72 A74 R79

Gene Ontology

	Molecular Function
GO:0000049	tRNA binding
GO:0003723	RNA binding
GO:0003735	structural constituent of ribosome
GO:0008097	5S rRNA binding
GO:0019843	rRNA binding

	Biological Process
GO:0000027	ribosomal large subunit assembly
GO:0002181	cytoplasmic translation
GO:0006412	translation

	Cellular Component
GO:0005737	cytoplasm
GO:0005829	cytosol
GO:0005840	ribosome
GO:0022625	cytosolic large ribosomal subunit
GO:1990904	ribonucleoprotein complex

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Molecular Function

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Biological Process

View graph for
Cellular Component

External links

PDB	RCSB:8ifb, PDBe:8ifb, PDBj:8ifb
PDBsum	8ifb
PubMed	38227611
UniProt	P62399\|RL5_ECOLI Large ribosomal subunit protein uL5 (Gene Name=rplE)

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