Structure of PDB 6ys3 Chain f

Receptor sequence

>6ys3f (length=177) Species: 469008 (Escherichia coli BL21(DE3))

AKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAIADKKLLD
NAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFE
RLITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRG
LDITITTTAKSDEEGRALLAAFDFPFR

3D structure

• PDB: 6ys3 Cryo-EM structure of the 50S ribosomal subunit at 2.58 Angstroms with modeled GBC SecM peptide
• Chain: f
• Resolution: 2.58 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	f	S24 M26 Q27 Q63 K64 L66 K88 T90 R92	S23 M25 Q26 Q62 K63 L65 K87 T89 R91
BS02	rna	f	K33 T35 N37 M38 G39 G41 T68 A70 R71 K72 F77 I85 K88 S121 F122 D123 R125 S129 G131 R150 G151 L152 D153 T155	K32 T34 N36 M37 G38 G40 T67 A69 R70 K71 F76 I84 K87 S120 F121 D122 R124 S128 G130 R149 G150 L151 D152 T154
BS03	rna	f	S73 V74 A75 R80	S72 V73 A74 R79

Gene Ontology

Molecular Function

• GO:0000049 tRNA binding
• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome
• GO:0008097 5S rRNA binding
• GO:0019843 rRNA binding

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:6ys3, PDBe:6ys3, PDBj:6ys3
• PDBsum: 6ys3
• UniProt: P62399|RL5_ECOLI Large ribosomal subunit protein uL5 (Gene Name=rplE)