Structure of PDB 7p5h Chain e

Receptor sequence

>7p5he (length=555) Species: 243274 (Thermotoga maritima MSB8)

MFKNAKEFVQYANKLKTLREKKLNGVSIYVCVGTGCTAKGALKVYSAFEE
ELKKRNLKVTLNRTGCCGRCSSGPLVKIMPYRFFYSNVAPEDVPEIVDRT
VLKGEPIERLFLTDPLTGEKVPRIEDTTLFKNQDFYIMEAIGESECDSIE
DYIARSGYESLVKALTSMTPEEIIETVKASGLRGRGGGGFPTGLKWEFTR
KAQGDIKFVVCNGDEGDPGAFMNRTLLERDPHLVLEGMIIAGYAVGAQKG
YAYIRAEYPFAVKMFKKAIEDARKLGLLGENILGTGFSFDLEVKEGAGAF
VCGEETALLASIEGKRGMPRPKPPFPAQSGLWGKPTLINNVETYANIPRI
LRDGVENYRKRGTENSPGTKMFSVAGPLKATGIIEVEFGTTLRDIIYNIC
GGFVEGEEFKAVQIGGPSGACLSEDFIDMPLDYDTLKKADAMVGSGGIVV
ITKKTCMVEVARFFLDFTKRESCGKCVPCREGTMQAYNILEKFTHGKATY
EDLKTLEHLSKTIKTASLCGLGKTAPNPILSTLKLFREEYIAHIEGECPS
GMCTA

3D structure

• PDB: 7p5h Structural insight on the mechanism of an electron-bifurcating [FeFe] hydrogenase.
• Chain: e
• Resolution: 2.3 Å

Enzymatic activity

• Enzyme Commision number: 1.12.1.4: hydrogenase (NAD(+), ferredoxin).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SF4	e	P331 S484 C485 G486 K487 C488 C491 L530 C531 L533 G534	P319 S472 C473 G474 K475 C476 C479 L518 C519 L521 G522
BS02	FMN	e	G196 G198 K207 N224 D226 G228 F312 G315 E317 N351 N352 G532 L533	G184 G186 K195 N212 D214 G216 F300 G303 E305 N339 N340 G520 L521
BS03	FES	e	C31 G33 T34 C36 C78 C79 R81 C82	C31 G33 T34 C36 C66 C67 R69 C70
BS04	ZN	e	C468 H555 C565	C456 H543 C553

Gene Ontology

Molecular Function

• GO:0008137 NADH dehydrogenase (ubiquinone) activity
• GO:0010181 FMN binding
• GO:0016491 oxidoreductase activity
• GO:0046872 metal ion binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Biological Process

• GO:1902600 proton transmembrane transport

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:7p5h, PDBe:7p5h, PDBj:7p5h
• PDBsum: 7p5h
• PubMed: 36018003
• UniProt: O52682|HYDB_THEMA Bifurcating [FeFe] hydrogenase beta subunit (Gene Name=hydB)