Structure of PDB 6uwi Chain d

Receptor sequence

>6uwid (length=645) Species: 1496 (Clostridioides difficile)

EDLDTDNDNIPDSYERNGYTIKDLIAVKWEDSFAEQGYKKYVSNYLESNT
AGDPYTDYEKASGSFDKAIKTEARDPLVAAYPIVGVGMEKLIISTNEHAS
TDQGKTVSRATTNSKTESNTAGVSVNVGNVTTNYSHTTDNSTAVQDSNGE
SWNTGLSINKGESAYINANVRYYNTGTAPMYKVTPTTNLVLDGDTLSTIK
AQENQIGNNLSPGDTYPKKGLSPLALNTMDQFSSRLIPINYDQLKKLETT
QVSGNFGTKNSSGQIVTEGNSWSDYISQIDSISASIILDTENESYERRVT
AKNLQDPEDKTPELTIGEAIEKAFGATKKDGLLYFNDIPIDESCVELIFD
DNTANKIKDSLKTLSDKKIYNVKLERGMNILIKTPTYFTNFDDYNNYPST
WSNVNTTNQDGLQGSANKLNGETKIKIPMSELKPYKRYVFSGYSKDPLTS
NSIIVKIKAKEEKTDYLVPEQGYTKFSYEFETTEKDSSNIEITLIGSGTT
YLDNLSITELNSTPEILDEPEVKIPTDQEIMDAHKIYFADLNFNPSTGNT
YINGMYFAPTQTNKEALDYIQKYRVEATLQYSGFKDIGTKDKEMRNYLGD
PNQPKTNYVNLRSYFTGGENIMTYKKLRIYAITPDDRELLVLSVD

3D structure

• PDB: 6uwi Structure of the cell-binding component of theClostridium difficilebinary toxin reveals a di-heptamer macromolecular assembly.
• Chain: d
• Resolution: 3.7 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CA	d	D222 D224 E231 N260 Y261 E263 D273	D6 D8 E15 N44 Y45 E47 D57
BS02	CA	d	D220 D222 D224 I226 E231 Y261	D4 D6 D8 I10 E15 Y45
BS03	CA	d	N621 D623 Q644 S646 D734	N390 D392 Q413 S415 D503
BS04	CA	d	D222 D224 E231 N260 E263 D273	D6 D8 E15 N44 E47 D57
BS05	CA	d	D220 D222 D224 I226 E231	D4 D6 D8 I10 E15
BS06	CA	d	N621 D623 Q644 S646 D734	N390 D392 Q413 S415 D503

Gene Ontology

Biological Process

• GO:0051260 protein homooligomerization

Cellular Component

• GO:0005576 extracellular region

External links

• PDB: RCSB:6uwi, PDBe:6uwi, PDBj:6uwi
• PDBsum: 6uwi
• PubMed: 31896582
• UniProt: O32739