Structure of PDB 6wdf Chain c

Receptor sequence

>6wdfc (length=209) Species: 83333 (Escherichia coli K-12)

MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDGYRAIQV
TTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVEL
FADVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQ
TPGKVFKGKKMAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSD
LIVKPAVKA

3D structure

• PDB: 6wdf Cryo-EM of elongating ribosome with EF-Tu•GTP elucidates tRNA proofreading.
• Chain: c
• Resolution: 3.3 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	c	K8 M11 R13 P23 D43 Y45 Q49 K56 N58 R59 T61 K62 P63 G66 H67 L79 W80 E81 R83 K114 G115 K116 G117 F118 G120 K123 R124 N126 F127 R128 T129 D131 A132 T133 H134 G135 N136 S137 L138 S139 H140 R141 V142 P143 S145 I146 G147 Q148 N149 Q150 T151 P152 K154 V155 F156 K157 G158 K159 M161 Q164 E168 R169 Q173 S174 L175 A192 V193 P194 T197 K208	K8 M11 R13 P23 D43 Y45 Q49 K56 N58 R59 T61 K62 P63 G66 H67 L79 W80 E81 R83 K114 G115 K116 G117 F118 G120 K123 R124 N126 F127 R128 T129 D131 A132 T133 H134 G135 N136 S137 L138 S139 H140 R141 V142 P143 S145 I146 G147 Q148 N149 Q150 T151 P152 K154 V155 F156 K157 G158 K159 M161 Q164 E168 R169 Q173 S174 L175 A192 V193 P194 T197 K208

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding
• GO:0019843 rRNA binding

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:6wdf, PDBe:6wdf, PDBj:6wdf
• PDBsum: 6wdf
• PubMed: 32612237
• UniProt: P60438|RL3_ECOLI Large ribosomal subunit protein uL3 (Gene Name=rplC)