Structure of PDB 6kwy Chain c

Receptor sequence

>6kwyc (length=1811) Species: 9606 (Homo sapiens)

RGFVPQKEIVYNKLLPYAERLDAESDLQLAQIKCNLGRAVQLQELWPGGL
FWTRKLSTYIRLYGRKFSKEDHVLFIKLLYELVSIPKLEISMMQGFARLL
INLLKKKELLSRADLELPWRPLYDMVERILYSKTEHLGLNWFPNSVENIL
KTLVKSCRPYFPADATAEMLEEWRPLMCPFDVTMQKAITYFEIFLPTSLP
PELHHKGFKLWFDELIGLWVSVQNLPQWEGQLVNLFARLATDNIGYIDWD
PYVPKIFTRILRSLNLPVGSSQVLVPRFLTNAYDIGHAVIWITAMMGGPS
KLVQKHLAGLFNSITSFYHPSNNGRWLNKLMKLLQRLPNSVVRRLHRERY
KKPSWLTPVPDSHKLTDQDVTDFVQCIIQPVLLAMFSKTGSLEAAQALQN
LALMRPELVIPPVLERTYPALETLTEPHQLTATLSCVIGVARSLVSGGRW
FPEGPTHMLPLLMRALPGVDPNDFSKCMITFQFIATFSTLVPLVDCSSVL
QERNDLTEVERELCSATAEFEDFVLQFMDRCFGLIESSTLEQTREETETE
KMTHLESLVELGLSSTFSTILTQCSKEIFMVALQKVFNFSTSHIFETRVA
GRMVADMCRAAVKCCPEESLKLFVPHCCSVITQLTMNDDVLNDEELDKEL
LWNLQLLSEITRVDGRKLLLYREQLVKILQRTLHLTCKQGYTLSCNLLHH
LLRSTTLIYPTEYCSVPGGFDKPPSEYFPIKDWGKPGDLWNLGIQWHVPS
SEEVSFAFYLLDSFLQPELVKLQHCGDGKLEMSRDDILQSLTIVHNCILG
SGNLLPPLKGEPVTNLVPSMVSLEETKLYTGLEYDLSRENHREVIATVIR
KLLNHILDNSEDDTKSLFLIIKIIGDLLQFQGSHKHEFDSRWKSFNLVKK
SMENRLHGKKQHIRALLIDRVMLQHELRTLTVEGCEYKKIHQDMIRDLLR
LSTSSYSQVRNKAQQTFFAALGAYNFCCRDIIPLVLEFLRPDRQGVTQQQ
FKGALYCLLGNHSGVCLANLHDWDCIVQTWPAIVSSGLSQAMSLEKPSIV
RLFDDLAEKIHRQYETIGLDFTIPKSCVEIAELLQQSKNPSINQILLSPE
KIKEGIKRQQEKNADALRNYENLVDTLLDGVEQRNLPWKFEHIGIGLLSL
LLRDDRVLPLRAIRFFVENLNHDAIVVRKMAISAVAGILKQLKRTHKKLT
INPCEISGCPKPTQIIAGDRPDNHWLHYDSKTIPRTKKEWESSCFVEKTH
WGYYTWPKNMVVYAGVEEQPKLGRSREDMTEAEQIIFDHFSDPKFVEQLI
TFLSLEDRKGKDKFNPRRFCLFKGIFRNFDDAFLPVLKPHLEHLVADSHE
STQRCVAEIIAGLIRGSKHWTFEKVEKLWELLCPLLRTALSNITVETYND
WGACIATSCESRDPRKLHWLFELLLESPLSGEGGSFVDACRLYVLQGGLA
QQEWRVPELLHRLLKYLEPKLTQVYKNVRERIGSVLTYIFMIDVSLPNTT
PTISPHVPEFTARILEKLKPLMDVDEEIQNHVDERTQGIKLLKTILKWLM
ASAGRSFSTAVTEQLQLLPLFFKIAPVENDNSYDELKRDAKLCLSLMSQG
LLYPHQVPLVLQVLKQTARSSSWHARYTVLTYLQTMVFYNLFIFLNNEDA
VKDIRWLVISLLEDEQLEVREMAATTLSGLLQCNFLTMDSPMQIHFEQLC
KTKLPKKRKRDPGSVGDTIPSAELVKRHAGVLGLGACVLSSPYDVPTWMP
QLLMNLSAHLNDPQPIEMTVKKTLSNFRRTHHDNWQEHKQQFTDDQLLVL
TDLLVSPCYYA

3D structure

• PDB: 6kwy Cryo-EM structures of the human PA200 and PA200-20S complex reveal regulation of proteasome gate opening and two PA200 apertures.
• Chain: c
• Resolution: 2.72 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	K0W	c	K30 Y34 K128 K129 W915 R928 K933 R943	K7 Y11 K105 K106 W892 R905 K910 R920
BS02	IHP	c	K156 K1213 K1216 H1219 K1271 Y1286 K1346 R1350 R1388 K1391 H1392 R1435	K133 K1190 K1193 H1196 K1248 Y1263 K1323 R1327 R1365 K1368 H1369 R1412

Gene Ontology

Molecular Function

• GO:0005515 protein binding
• GO:0016504 peptidase activator activity
• GO:0070577 lysine-acetylated histone binding
• GO:0070628 proteasome binding

Biological Process

• GO:0006281 DNA repair
• GO:0006974 DNA damage response
• GO:0007283 spermatogenesis
• GO:0010499 proteasomal ubiquitin-independent protein catabolic process
• GO:0030154 cell differentiation
• GO:0035092 sperm DNA condensation

Cellular Component

• GO:0000502 proteasome complex
• GO:0005634 nucleus
• GO:0005654 nucleoplasm
• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0016607 nuclear speck
• GO:1990111 spermatoproteasome complex

External links

• PDB: RCSB:6kwy, PDBe:6kwy, PDBj:6kwy
• PDBsum: 6kwy
• PubMed: 32134919
• UniProt: Q14997|PSME4_HUMAN Proteasome activator complex subunit 4 (Gene Name=PSME4)