Structure of PDB 8uqm Chain b

Receptor sequence

>8uqmb (length=76) Species: 562 (Escherichia coli)

TRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTKFHAGANVGCGRDHTL
FAKADGKVKFEVKGPKNRKFISIEAE

3D structure

• PDB: 8uqm Escherichia coli transcription-translation coupled complex class A (TTC-A) containing RfaH bound to ops signal, mRNA with a 21 nt long spacer, and fMet-tRNAs in E-site and P-site of the ribosome
• Chain: b
• Resolution: 5.3 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	b	N12 R14 D15 S16 E17 A18 K19 R20 K24 F26 G27 E29 L32 A33 G34 S35 I36 R39 R41 G42 T43 K44 F45 H46 G54 R55 D56 H57 T58 F60 F69 K75 R77 K78	N3 R5 D6 S7 E8 A9 K10 R11 K15 F17 G18 E20 L23 A24 G25 S26 I27 R30 R32 G33 T34 K35 F36 H37 G45 R46 D47 H48 T49 F51 F60 K66 R68 K69
BS02	rna	b	G73 P74 N76	G64 P65 N67

Gene Ontology

Molecular Function

• GO:0000049 tRNA binding
• GO:0003735 structural constituent of ribosome
• GO:0005515 protein binding
• GO:0019843 rRNA binding
• GO:0043022 ribosome binding

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0001558 regulation of cell growth
• GO:0002181 cytoplasmic translation
• GO:0006412 translation
• GO:0042256 cytosolic ribosome assembly
• GO:0090070 positive regulation of ribosome biogenesis
• GO:1902626 assembly of large subunit precursor of preribosome

Cellular Component

• GO:0005737 cytoplasm
• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8uqm, PDBe:8uqm, PDBj:8uqm
• PDBsum: 8uqm
• PubMed: 39117885
• UniProt: P0A7L8|RL27_ECOLI Large ribosomal subunit protein bL27 (Gene Name=rpmA)