Structure of PDB 7p8n Chain b

Receptor sequence

>7p8nb (length=560) Species: 243274 (Thermotoga maritima MSB8)

MFKNAKEFVQYANKLKTLREKKLNGVSIYVCVGTGCTAKGALKVYSAFEE
ELKKRNLLKVTLNRTGCCGRCSSGPLVKIMPYRFFYSNVAPEDVPEIVDR
TVLKGEPIERLFLTDPLTGEKVPRIEDTTLFKNQDFYIMEAIGESECDSI
EDYIARSGYESLVKALTSMTPEEIIETVKASGLRGRGGGGFPTGLKWEFT
RKAQGDIKFVVCNGDEGDPGAFMNRTLLERDPHLVLEGMIIAGYAVGAQK
GYAYIRAEYPFAVKMFKKAIEDARKLGLLGENILGTGFSFDLEVKEGAGA
FVCGEETALLASIEGKRGMPRPKPPFPAQSGLWGKPTLINNVETYANIPR
ILRDGVENYRKRGTENSPGTKMFSVAGPLKATGIIEVEFGTTLRDIIYNI
CGGFVEGEEFKAVQIGGPSGACLSEDFIDMPLDYDTLKKADAMVGSGGIV
VITKKTCMVEVARFFLDFTKRESCGKCVPCREGTMQAYNILEKFTHGKAT
YEDLKTLEHLSKTIKTASLCGLGKTAPNPILSTLKLFREEYIAHIEGECP
SGMCTAFKKY

3D structure

• PDB: 7p8n Structural insight on the mechanism of an electron-bifurcating [FeFe] hydrogenase.
• Chain: b
• Resolution: 2.8 Å

Enzymatic activity

• Enzyme Commision number: 1.12.1.4: hydrogenase (NAD(+), ferredoxin).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SF4	b	P331 S484 C485 K487 C488 C491 L530 C531 L533 G534	P320 S473 C474 K476 C477 C480 L519 C520 L522 G523
BS02	FMN	b	G198 K207 D226 E227 G228 F312 G315 E316 E317 N351 N352 G532 L533	G187 K196 D215 E216 G217 F301 G304 E305 E306 N340 N341 G521 L522
BS03	FES	b	C31 G33 T34 C36 C78 C79 R81 C82	C31 G33 T34 C36 C67 C68 R70 C71
BS04	ZN	b	C468 H555 C560	C457 H544 C549

Gene Ontology

Molecular Function

• GO:0008137 NADH dehydrogenase (ubiquinone) activity
• GO:0010181 FMN binding
• GO:0016491 oxidoreductase activity
• GO:0046872 metal ion binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Biological Process

• GO:1902600 proton transmembrane transport

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:7p8n, PDBe:7p8n, PDBj:7p8n
• PDBsum: 7p8n
• PubMed: 36018003
• UniProt: O52682|HYDB_THEMA Bifurcating [FeFe] hydrogenase beta subunit (Gene Name=hydB)