Structure of PDB 6wdl Chain b

Receptor sequence

>6wdlb (length=271) Species: 83333 (Escherichia coli K-12) [Search protein sequence]

AVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRNNNGRITT
RHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGER
RYILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKG
GQLARSAGTYVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHM
LRVLGKAGAARWRGVRPTVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQ
TKGKKTRSNKRTDKFIVRRRS

3D structure

PDB

6wdl Cryo-EM of elongating ribosome with EF-Tu•GTP elucidates tRNA proofreading.

Chain

Resolution

3.7 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	b	R174 M200 R268	R174 M200 R268
BS02	rna	b	P7 S9 P10 G11 R12 R13 H14 K27 K35 K38 S39 G41 R42 N43 N44 N45 R47 I48 T49 T50 R51 H52 G55 H57 K58 Q59 A60 Y61 R62 I73 Y82 R86 L94 D97 R100 K146 G148 K149 Q152 A154 R155 S156 A157 G158 T159 Y170 L175 R176 S177 E179 R181 A197 H199 M200 L204 G205 K206 A207 R211 W212 R216 P217 T218 V219 R220 G221 T222 A223 M224 N225 P226 V227 D228 H231 G234 E235 G236 R237 N238 F239 G240 K241 H242 T245 P246 W247 K252 G253 K254 K255 T256 R257 K260 R261 R270	P7 S9 P10 G11 R12 R13 H14 K27 K35 K38 S39 G41 R42 N43 N44 N45 R47 I48 T49 T50 R51 H52 G55 H57 K58 Q59 A60 Y61 R62 I73 Y82 R86 L94 D97 R100 K146 G148 K149 Q152 A154 R155 S156 A157 G158 T159 Y170 L175 R176 S177 E179 R181 A197 H199 M200 L204 G205 K206 A207 R211 W212 R216 P217 T218 V219 R220 G221 T222 A223 M224 N225 P226 V227 D228 H231 G234 E235 G236 R237 N238 F239 G240 K241 H242 T245 P246 W247 K252 G253 K254 K255 T256 R257 K260 R261 R270

Gene Ontology

	Molecular Function
GO:0003723	RNA binding
GO:0003735	structural constituent of ribosome
GO:0005515	protein binding
GO:0008270	zinc ion binding
GO:0016740	transferase activity
GO:0019843	rRNA binding

	Biological Process
GO:0000027	ribosomal large subunit assembly
GO:0002181	cytoplasmic translation
GO:0006412	translation
GO:0032297	negative regulation of DNA-templated DNA replication initiation

	Cellular Component
GO:0005737	cytoplasm
GO:0005829	cytosol
GO:0005840	ribosome
GO:0015934	large ribosomal subunit
GO:0022625	cytosolic large ribosomal subunit
GO:1990082	DnaA-L2 complex
GO:1990904	ribonucleoprotein complex

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:6wdl, PDBe:6wdl, PDBj:6wdl
PDBsum	6wdl
PubMed	32612237
UniProt	P60422\|RL2_ECOLI Large ribosomal subunit protein uL2 (Gene Name=rplB)

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