Structure of PDB 7ade Chain a

Receptor sequence

>7adea (length=417) Species: 562 (Escherichia coli)

MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAKSGEDIF
GDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGK
IRPPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERG
NGSTEDLTARVLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHP
DCVLMVLLIDERPEEVTEMQRLVKGEVVASTFDEPASRHVQVAEMVIEKA
KRLVEHKKDVIILLDSITRLARAYNTVVPASGKVLTGGVDANALHRPKRF
FGAARNVEEGGSLTIIATALIDTGSKMDEVIYEEFKGTGNMELHLSRKIA
EKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGEIDAMEFLI
NKLAMTKTNDDFFEMMK

3D structure

• PDB: 7ade Steps toward translocation-independent RNA polymerase inactivation by terminator ATPase rho.
• Chain: a
• Resolution: 4.2 Å

Enzymatic activity

• Enzyme Commision number: 3.6.4.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	a	F36 P76 Y80 S82 P83 E108 R109 Y110	F36 P76 Y80 S82 P83 E108 R109 Y110
BS02	ADP	a	A182 G183 K184 T185 M186 R353 F355	A182 G183 K184 T185 M186 R353 F355
BS03	BEF	a	K184 R212	K184 R212

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003723 RNA binding
• GO:0004386 helicase activity
• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0008186 ATP-dependent activity, acting on RNA
• GO:0016787 hydrolase activity
• GO:0016887 ATP hydrolysis activity
• GO:0042802 identical protein binding

Biological Process

• GO:0006353 DNA-templated transcription termination

Cellular Component

• GO:0005829 cytosol
• GO:0016020 membrane

External links

• PDB: RCSB:7ade, PDBe:7ade, PDBj:7ade
• PDBsum: 7ade
• PubMed: 33243850
• UniProt: P0AG30|RHO_ECOLI Transcription termination factor Rho (Gene Name=rho)