Structure of PDB 8qbl Chain Z

Receptor sequence

>8qblZ (length=309) Species: 469008 (Escherichia coli BL21(DE3)) [Search protein sequence]

SAEYLNTFRLRNLGLPVMNNLHDMSKATRISVETLRLLIYTADFRYRIYT
VEKKGPEKRMRTIYQPSRELKALQGWVLRNILDKLSSSPFSIGFEKHQSI
LNNATPHIGANFILNIDLEDFFPSLTANKVFGVFHSLGYNRLISSVLTKI
CCYKNLLPQGAPSSPKLANLICSKLDYRIQGYAGSRGLIYTRYADDLTLS
AQSMKKVVKARDFLFSIIPSEGLVINSKKTCISGPRSQRKVTGLVISQEK
VGIGREKYKEIRAKIHHIFCGKSSEIEHVRGWLSFILSVDSKSHRRLITY
ISKLEKKYG

3D structure

PDB

8qbl Retron-Eco1 assembles NAD + -hydrolyzing filaments that provide immunity against bacteriophages.

Chain

Resolution

2.66 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

2.7.7.49: RNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	Z	K56 F96 E97 Q100 S101 G162 A163 P164 K230 C233 R238 S239 Q240 R257 K261 A265 S286 S290	K54 F94 E95 Q98 S99 G160 A161 P162 K228 C231 R236 S237 Q238 R255 K259 A263 S284 S288
BS02	dna	Z	E35 L39 Y42 F46 Q67 P68 A129 K131 S147 L172 I173 S175 K176 Y179 Y184 S218 T244 G283 W284	E33 L37 Y40 F44 Q65 P66 A127 K129 S145 L170 I171 S173 K174 Y177 Y182 S216 T242 G281 W282

Gene Ontology

	Molecular Function
GO:0003723	RNA binding
GO:0003964	RNA-directed DNA polymerase activity
GO:0046872	metal ion binding

	Biological Process
GO:0006974	DNA damage response
GO:0051607	defense response to virus

View graph for
Molecular Function

View graph for
Biological Process

External links

PDB	RCSB:8qbl, PDBe:8qbl, PDBj:8qbl
PDBsum	8qbl
PubMed	38788717
UniProt	P23070\|RT86_ECOLX Retron Ec86 reverse transcriptase (Gene Name=ret)

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