Structure of PDB 4rvz Chain Z

Receptor sequence

>4rvzZ (length=420) Species: 224325 (Archaeoglobus fulgidus DSM 4304)

MRVWVGIDDTDSSRGMCTTYLAVLAMERVERELGKVIGFPRLIRLNPTIP
YKTRGNGAVSFLVEVDDVGELVDVVNEVIIEHAMLDDEKTNPGAVFVDEE
LAVKLKPFADKAIKDVLQIDEALFVIGKYFIPHLRHKKGRGLIGALAAVG
AELEDFTLELIAYRYPERFGTEREYDEESFFDMDYELYPQTFDNVDWCND
VVVCIPNTPCPVLYGIRGESVEALYKAMESVKTEPVDRRMIFVTNHATDM
HLIGEEEVHRLENYRSYRLRGRVTLEPYDIEGGHVFFEIDTKFGSVKCAA
FEPTKQFRNVIRLLRKGDVVEVYGSMKKDTINLEKIQIVELAEIWVEKNP
ICPSCGRRMESAGRGQGFRCKKCRTKADEKLREKVERELQPGFYEVPPSA
RRHLSKPLIRMNVEGRHIFR

3D structure

• PDB: 4rvz A covalent approach for site-specific RNA labeling in Mammalian cells.
• Chain: Z
• Resolution: 2.9 Å

Enzymatic activity

• Enzyme Commision number: 6.3.4.22: tRNA(Ile)(2)-agmatinylcytidine synthase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	Z	C352 C355	C352 C355
BS02	ACP	Z	D8 D9 D11 T18 L45 G55 N56 G57 A112 V116 L117 R140 G141 I143 G144	D8 D9 D11 T18 L45 G55 N56 G57 A112 V116 L117 R140 G141 I143 G144
BS03	MG	Z	D9 D11 T90	D9 D11 T90
BS04	MG	Z	D8 T18 G57 A58	D8 T18 G57 A58
BS05	ZNN	Z	V203 C204 R217 S399	V203 C204 R217 S399

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0005524 ATP binding
• GO:0016874 ligase activity
• GO:0016879 ligase activity, forming carbon-nitrogen bonds

Biological Process

• GO:0002101 tRNA wobble cytosine modification
• GO:0008033 tRNA processing

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:4rvz, PDBe:4rvz, PDBj:4rvz
• PDBsum: 4rvz
• PubMed: 25694369
• UniProt: O28025|TIAS_ARCFU tRNA(Ile2) 2-agmatinylcytidine synthetase TiaS (Gene Name=tiaS)