Structure of PDB 1p8k Chain Z

Receptor sequence

>1p8kZ (length=254) Species: 162425 (Aspergillus nidulans) [Search protein sequence]

GSDLTYAYLVGLFEGDGYFSITKKGKYLTYELGIELSIKDVQLIYKIKKI
LGIGIVSFRKRNEIEMVALRIRDKNHLKSFILPIFEKYPMFSNKQYDYLR
FRNALLSGIISLEDLPDYTRSDEPLNSIESIINTSYFSAWLVGFIEAEGC
FSVYKLNKDDDYLIASFDIAQRDGDILISAIRKYLSFTTKVYLDKTNCSK
LKVTSVRSVENIIKFLQNAPVKLLGNKKLQYLLWLKQLRKISRYSEKIKI
PSNY

3D structure

PDB

1p8k Structural and biochemical analyses of DNA and RNA binding by a bifunctional homing endonuclease and group I intron splicing factor.

Chain

Resolution

2.6 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	Z	K24 Y27 I55 R59 R61 R70 R72 K74 E148 Q171 R172	K24 Y27 I55 R59 R61 R70 R72 K74 E148 Q171 R172	PDBbind-CN: Kd=11nM
BS02	dna	Z	D16 A147 E148 C150 S152 Y154 D168 K200 K202 K227 Q230	D16 A147 E148 C150 S152 Y154 D168 K200 K202 K227 Q230	PDBbind-CN: Kd=11nM
BS03	dna	Z	D16 E35 L36 S37 M66 Y162 K190 Y192 K200 T204 S205 V206 R243	D16 E35 L36 S37 M66 Y162 K190 Y192 K200 T204 S205 V206 R243	PDBbind-CN: Kd=11nM
BS04	dna	Z	D16 G17 Y18 S20 T22 K23 E35 K94	D16 G17 Y18 S20 T22 K23 E35 K94	PDBbind-CN: Kd=11nM
BS05	MG	Z	G15 E148	G15 E148

Gene Ontology

	Molecular Function
GO:0004519	endonuclease activity

View graph for
Molecular Function

External links

PDB	RCSB:1p8k, PDBe:1p8k, PDBj:1p8k
PDBsum	1p8k
PubMed	14633971
UniProt	P03880\|ANI1_EMEND Intron-encoded DNA endonuclease I-AniI (Gene Name=I-AniI)

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