Structure of PDB 3nho Chain X

Receptor sequence

>3nhoX (length=425) Species: 29320 (Paenarthrobacter nicotinovorans)

MYDAIVVGGGFSGLKAARDLTNAGKKVLLLEGGERLGGRAYSRESRNVPG
LRVEIGGAYLHRKHHPRLAAELDRYGIPTAAASEFTSFRHRLGPTAVDQA
FPIPGSEAVAVEAATYTLLRDAHRIDLEKGLENQDLEDLDIPLNEYVDKL
DLPPVSRQFLLAWAWNMLGQPADQASALWMLQLVAAHHYSILGVVLSLDE
VFSNGSADLVDAMSQEIPEIRLQTVVTGIDQSGDVVNVTVKDGHAFQAHS
VIVATPMNTWRRIVFTPALPERRRSVIEEGHGGQGLKILIHVRGAEAGIE
CVGDGIFPTLYDYCEVSESERLLVAFTDSGSFDPTDIGAVKDAVLYYLPE
VEVLGIDYHDWIADPLFEGPWVAPRVGQFSRVHKELGEPAGRIHFVGSDV
SLEFPGYIEGALETAECAVNAILHS

3D structure

• PDB: 3nho Crystal structure analysis of free and substrate-bound 6-hydroxy-L-nicotine oxidase from Arthrobacter nicotinovorans.
• Chain: X
• Resolution: 2.85 Å

Enzymatic activity

• Enzyme Commision number: 1.5.3.5: (S)-6-hydroxynicotine oxidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	HNM	X	M167 W179 L183 F326 W371	M167 W179 L183 F326 W371
BS02	FAD	X	V7 G8 G10 S12 E31 G32 G38 R39 G56 G57 A58 Y59 V226 T255 P256 L366 F367 P370 W371 G397 S398 G406 Y407 I408	V7 G8 G10 S12 E31 G32 G38 R39 G56 G57 A58 Y59 V226 T255 P256 L366 F367 P370 W371 G397 S398 G406 Y407 I408
BS03	GP7	X	Q99 T115 F159 L160 M180 V195	Q99 T115 F159 L160 M180 V195

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0016491 oxidoreductase activity
• GO:0018531 (S)-6-hydroxynicotine oxidase activity

Biological Process

• GO:0009820 alkaloid metabolic process
• GO:0019608 nicotine catabolic process

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:3nho, PDBe:3nho, PDBj:3nho
• PDBsum: 3nho
• PubMed: 20006620
• UniProt: Q93NH4|HLNO_PAENI (S)-6-hydroxynicotine oxidase (Gene Name=6-hlno)