Structure of PDB 3jb9 Chain X

Receptor sequence

>3jb9X (length=1195) Species: 284812 (Schizosaccharomyces pombe 972h-)

KKKQIINYANSNWGEGVEFQETMLQCILEAVIVSAKQVLSLLHELKLFEN
FLWQRVNTEMSLNHINLTCMLLLYKSKYEYITWDLINRFQLFFEKVIEVS
LSLNLSEVVYMIQFITLCFQFSNIEKLRKLVYQLTNISILNSLLDKVKYL
LHDSSSLTKAFDSYKEKRPVEKFPLHNLLSRWIHSLLIKSISYAQKQEAK
VTPLLAIINMSLVLLSAFPTRRFAHPVIEDSCFYTALRMSLYYDSNELFK
KMTDDLNYVLKFPFDNTRGNEYEKEQKIRNDELVYYHLQLTLFSDFQLQQ
RQKLEEITSFLSFNSLKSLCSKCYLRTSFPEKYAIKVDFEFLKNVFINTY
DRRLVNDYDEIINFTLKDVLGERSVMDQENSLTNTAIQYLSISFFMRQQS
KAYKKLLLRSLYAELLNFSEQYRRLSIKNATKNLNFFSLNNFKVTSVAPL
PQFVKCQMGLSRPSALRDLKNSSPFLCLIYISKDMEYKLLHGNALDPLEV
TDFTIATICNDDVGMFMQSDSDNKSINVYLSPFYYHSLAGLGEYRPKQLK
FNFALVLSNKYWLDLNILVSLLNRAKEFPKWFEDLFLGFGTPDICAFPNA
NSIYARNLFNTVEQLQSVLPNCHVPSNLSTESLLIKFYTNQNKSADVTAS
DRHFSNRLYTYNDKQLESILRGSQPGLTMVNGPTRCGKHVLVCKLLEVLQ
DTSPNDRTVVLSDSNFSMNTLFTLLEKARCFHHLLYLSDEGTLERYGTLS
SWISKLPGLLREIGRLAASIQAPGSHDASPDTALYFRDAYIKRLWEKYLN
TVDKDSVDAYNRFPFHSYFGDKSKRPIETYNKDNFFDYATKLYGELEYMF
QQLEEIRPFGLLRYYEDQELYALCQQSRIIGCTWTSLSTRLGTLKEKGFC
FNNLIVMNSQNISESSITSILLSNCEPTGFDRLVLLGNQYNGSLFKRLRY
LKSRIIDLNTQYNVRESISSLCSSIYPLDIKTVDSSPNKRLDYGNSGFAH
EVQFINVGAFKGSQETEPVSGYKQNLGEAEYAVALFQYMRMLGYPTNEIV
ICTLYESQVSLLNEIISVRCSHNSFFGQPAFVGTVEKLPKRVNFVIFTTV
ESKEASDHWNPKTFYKAFSACSYGLYVLCNRDLFRSTRGLEKLWNEIEKT
PDKLLLTTGEIYPSSHSSVETFEIENLLHLSNYVVEMTKKRLNTN

3D structure

• PDB: 3jb9 Structure of a yeast spliceosome at 3.6-angstrom resolution
• Chain: X
• Resolution: 3.6 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ADP	X	Y725 T726 R751 C752 G753 H755 Y1046 T1066	Y659 T660 R685 C686 G687 H689 Y962 T982

Gene Ontology

Molecular Function

• GO:0003729 mRNA binding
• GO:0005515 protein binding

Biological Process

• GO:0000398 mRNA splicing, via spliceosome
• GO:0006397 mRNA processing
• GO:0008380 RNA splicing
• GO:0045292 mRNA cis splicing, via spliceosome

Cellular Component

• GO:0000974 Prp19 complex
• GO:0005634 nucleus
• GO:0005681 spliceosomal complex
• GO:0005684 U2-type spliceosomal complex
• GO:0071013 catalytic step 2 spliceosome
• GO:0071014 post-mRNA release spliceosomal complex

External links

• PDB: RCSB:3jb9, PDBe:3jb9, PDBj:3jb9
• PDBsum: 3jb9
• PubMed: 26292707
• UniProt: O94508|CWF11_SCHPO Pre-mRNA-splicing factor cwf11 (Gene Name=cwf11)