Structure of PDB 3h1c Chain X

Receptor sequence

>3h1cX (length=544) Species: 562 (Escherichia coli)

MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTVVGQKKA
KPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIR
PLFPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIG
AARVGYINDQYVLNPTQDELKESKLDLVVAGTEAAVLMVESEAQLLSEDQ
MLGAVVFGHEQQQVVIQNINELVKEAGKPRWDWQPEPVNEALNARVAALA
EARLSDAYRITDKQERYAQVDVIKSETIATLLAEDETLDENELGEILHAI
EKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVLPRTHGSALFTRGETQA
LVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGMVGSPKRRE
IGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRD
GISALQMDIKIEGITKEIMQVALNQAKGARLHILGVMEQAINAP

3D structure

• PDB: 3h1c Crystal structure of Escherichia coli polynucleotide phosphorylase core bound to RNase E, RNA and manganese: implications for catalytic mechanism and RNA degradosome assembly
• Chain: X
• Resolution: 3.57 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.8: polyribonucleotide nucleotidyltransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	X	E19 S34	E19 S34
BS02	peptide	X	D322 M323 I324 G326 L327 V329 R330 T331 G332 V333 P335 G528 V536 A540	D322 M323 I324 G326 L327 V329 R330 T331 G332 V333 P335 G528 V536 A540
BS03	WO4	X	R93 R399 H403 D508	R93 R399 H403 D508

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0004654 polyribonucleotide nucleotidyltransferase activity

Biological Process

• GO:0006396 RNA processing
• GO:0006402 mRNA catabolic process

External links

• PDB: RCSB:3h1c, PDBe:3h1c, PDBj:3h1c
• PDBsum: 3h1c
• PubMed: 19327365
• UniProt: P05055|PNP_ECOLI Polyribonucleotide nucleotidyltransferase (Gene Name=pnp)