Structure of PDB 3blp Chain X

Receptor sequence

>3blpX (length=496) Species: 9606 (Homo sapiens)

QYSSNTQQGRTSIVHLFEWRWVDIALECERYLAPKGFGGVQVSPPNENVA
IHNPFRPWWERYQPVSYKLCTRSGNEDEFRNMVTRCNNVGVRIYVDAVIN
HMCGNAVSAGTSSTCGSYFNPGSRDFPAVPYSGWDFNDGKCKTGSGDIEN
YNDATQVRDCRLSGLLDLALGKDYVRSKIAEYMNHLIDIGVAGFRIDASK
HMWPGDIKAILDKLHNLNSNWFPEGSKPFIYQEVIDLGGEPIKSSDYFGN
GRVTEFKYGAKLGTVIRKWNGEKMSYLKNWGEGWGFMPSDRALVFVDNHD
NQRGHGAGGASILTFWDARLYKMAVGFMLAHPYGFTRVMSSYRWPRYFEN
GKDVNDWVGPPNDNGVTKEVTINPDTTCGNDWVCEHRARQIRNMVNFRNV
VDGQPFTNWYDNGSNQVAFGRGNRGFIVFNNDDWTFSLTLQTGLPAGTYC
DVISGDKINGNCTGIKIYVSDDGKAHFSISNSAEDPFIAIHAESKL

3D structure

• PDB: 3blp Structure-function relationships in human salivary alpha-amylase: role of aromatic residues in a secondary binding site
• Chain: X
• Resolution: 1.6 Å

Enzymatic activity

• Enzyme Commision number: 3.2.1.1: alpha-amylase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	GLC	X	W58 W59 Q63 H305	W58 W59 Q63 H305
BS02	GLC	X	K200 G306	K200 G306
BS03	AGL	X	H201 E233 G306	H201 E233 G306
BS04	CA	X	N100 R158 D167 H201	N100 R158 D167 H201
BS05	HMC	X	W58 Y62 H101 D197 E233 H299 D300	W58 Y62 H101 D197 E233 H299 D300

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0004556 alpha-amylase activity
• GO:0005509 calcium ion binding
• GO:0016160 amylase activity
• GO:0016798 hydrolase activity, acting on glycosyl bonds
• GO:0031404 chloride ion binding
• GO:0043169 cation binding
• GO:0046872 metal ion binding

Biological Process

• GO:0005975 carbohydrate metabolic process
• GO:0009311 oligosaccharide metabolic process

Cellular Component

• GO:0005576 extracellular region
• GO:0005615 extracellular space
• GO:0070062 extracellular exosome

External links

• PDB: RCSB:3blp, PDBe:3blp, PDBj:3blp
• PDBsum: 3blp
• UniProt: P0DUB6|AMY1A_HUMAN Alpha-amylase 1A (Gene Name=AMY1A)