Structure of PDB 3b0u Chain X

Receptor sequence
>3b0uX (length=312) Species: 300852 (Thermus thermophilus HB8) [Search protein sequence]
LDPRLSVAPMVDRTDRHFRFLVRQVSLGVRLYTEMTVDQAVLRGNRERLL
AFRPEEHPIALQLAGSDPKSLAEAARIGEAFGYDEINLNLGCPSEKAQEG
GYGACLLLDLARVREILKAMGEAVRVPVTVKMRLGLEGKETYRGLAQSVE
AMAEAGVKVFVVHARSALLALSTKANREIPPLRHDWVHRLKGDFPQLTFV
TNGGIRSLEEALFHLKRVDGVMLGRAVYEDPFVLEEADRRVFGLPRRPSR
LEVARRMRAYLEEEVLKGTPPWAVLRHMLNLFRGRPKGRLWRRLLSEGRS
LQALDRALRLME
3D structure
PDB3b0u Molecular basis of dihydrouridine formation on tRNA
ChainX
Resolution1.948 Å
3D
structure
[Spin on]
[Spin off]
[Reset orientation]

[High quality]
[Low quality]

[White background]
[Black background]

[Download]
[Download structure with residue number starting from 1]
Enzymatic activity
Enzyme Commision number 1.3.1.-
1.3.1.91: tRNA-dihydrouridine(20) synthase [NAD(P)(+)].
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 rna X M36 V38 A41 G45 N46 R49 L50 C93 S95 K97 T174 K175 R178 M35 V37 A40 G44 N45 R48 L49 C92 S94 K96 T173 K174 R177
BS02 FMN X P10 M11 V12 M36 Q63 N90 K132 H164 R178 N203 G205 G225 R226 P9 M10 V11 M35 Q62 N89 K131 H163 R177 N202 G204 G224 R225
Gene Ontology
Molecular Function
GO:0000049 tRNA binding
GO:0000166 nucleotide binding
GO:0010181 FMN binding
GO:0016491 oxidoreductase activity
GO:0017150 tRNA dihydrouridine synthase activity
GO:0050660 flavin adenine dinucleotide binding
GO:0102264 tRNA-dihydrouridine20 synthase activity
GO:0102266 tRNA-dihydrouridine20a synthase activity
Biological Process
GO:0002943 tRNA dihydrouridine synthesis
GO:0008033 tRNA processing

View graph for
Molecular Function
View graph for
Biological Process
External links
PDB RCSB:3b0u, PDBe:3b0u, PDBj:3b0u
PDBsum3b0u
PubMed22123979
UniProtQ5SMC7|DUSAL_THET8 tRNA-dihydrouridine(20/20a) synthase (Gene Name=dus)

[Back to BioLiP]