Structure of PDB 1z2o Chain X

Receptor sequence

>1z2oX (length=311) Species: 294381 (Entamoeba histolytica HM-1:IMSS)

QTVSLFIWLPESKQKTLFISTKNHTQFELNNIIFDVTLSTELPDKEPNAI
ITKRTHPVGKMADEMRKYEKDHPKVLFLESSAIHDMMSSREEINALLIKN
NIPIPNSFSVKSKEEVIQLLQSKQLILPFIVKPENAQGTFNAHQMKIVLE
QEGIDDIHFPCLCQHYINHNNKIVKVFCIGNTLKWQTRTSLPNVHRCGIK
SVDFNNQHLEDILSWPEGVIDKQDIIENSANRFGSKILEDPILLNLTSEA
EMRDLAYKVRCALGVQLCGIDFIKENEQGNPLVVDVNVFPSYGGKVDFDW
FVEKVALCYTE

3D structure

• PDB: 1z2o Specificity determinants in inositol polyphosphate synthesis: crystal structure of inositol 1,3,4-trisphosphate 5/6-kinase.
• Chain: X
• Resolution: 1.24 Å

Enzymatic activity

• Enzyme Commision number: 2.7.1.134: inositol-tetrakisphosphate 1-kinase.
  2.7.1.159: inositol-1,3,4-trisphosphate 5/6-kinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MG	X	D275 D289	D271 D285
BS02	MG	X	D289 N291	D285 N287
BS03	ADP	X	R94 K136 H147 M149 Q168 Y170 I171 H173 S194 L195 N210 V288 D289	R90 K132 H143 M145 Q164 Y166 I167 H169 S190 L191 N206 V284 D285
BS04	I4P	X	K17 Q141 G142 H147 K179 N291 S295	K13 Q137 G138 H143 K175 N287 S291

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0005524 ATP binding
• GO:0016301 kinase activity
• GO:0016853 isomerase activity
• GO:0046872 metal ion binding
• GO:0047325 inositol-3,4,5,6-tetrakisphosphate 1-kinase activity
• GO:0052725 inositol-1,3,4-trisphosphate 6-kinase activity
• GO:0052726 inositol-1,3,4-trisphosphate 5-kinase activity

Biological Process

• GO:0016310 phosphorylation
• GO:0032957 inositol trisphosphate metabolic process

External links

• PDB: RCSB:1z2o, PDBe:1z2o, PDBj:1z2o
• PDBsum: 1z2o
• PubMed: 15837423
• UniProt: Q9XYQ1|ITPK1_ENTH1 Inositol-tetrakisphosphate 1-kinase (Gene Name=ITPK1)