Structure of PDB 1mw8 Chain X

Receptor sequence
>1mw8X (length=561) Species: 562 (Escherichia coli) [Search protein sequence]
GKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGDERGALVNRM
GVDPWHNWEAHYEVLPGKEKVVSELKQLAEKADHIYLATDLDREGEAIAW
HLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNIDRVNAQQARRFM
DRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEEFWE
VDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLER
EDKPTTSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMR
TDSTNLSQDAVNMVRGYISDNFGKKYLPESPNQYASEAREAIRPSDVNVM
AESLKDMEADAQKLYQLIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARG
RILRFDGWTKVMPAEDRILPAVNKGDALTLVELTPAQHFTKPPARFSEAS
LVKELEKRGIGRPSTYASIISTIQDRGYVRVENRRFYAEKMGEIVTDRLE
ENFRELMNYDFTAQMENNLDQVANHEAEWKAVLDHFFSDFTQQLDKAEKD
PEEGGMRPNQM
3D structure
PDB1mw8 Structure of a Complex between E. coli DNA Topoisomerase I and Single-Stranded DNA.
ChainX
Resolution1.9 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) E9 S10 D113 E115 Y319 R321
Catalytic site (residue number reindexed from 1) E8 S9 D92 E94 Y298 R300
Enzyme Commision number 5.6.2.1: DNA topoisomerase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna X E115 R169 D172 R173 G176 Y177 S180 W184 R189 G190 S192 A193 G194 V196 Q197 R321 T496 R507 E94 R148 D151 R152 G155 Y156 S159 W163 R168 G169 S171 A172 G173 V175 Q176 R300 T465 R476
BS02 TMP X D113 R114 E115 R161 R168 R365 S495 D92 R93 E94 R140 R147 R339 S464
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003916 DNA topoisomerase activity
GO:0003917 DNA topoisomerase type I (single strand cut, ATP-independent) activity
Biological Process
GO:0006265 DNA topological change

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Molecular Function
View graph for
Biological Process
External links
PDB RCSB:1mw8, PDBe:1mw8, PDBj:1mw8
PDBsum1mw8
PubMed14604525
UniProtP06612|TOP1_ECOLI DNA topoisomerase 1 (Gene Name=topA)

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