Structure of PDB 8eu9 Chain W

Receptor sequence

>8eu9W (length=442) Species: 559292 (Saccharomyces cerevisiae S288C) [Search protein sequence]

SLIAAHSHITGLGLDENLQPRPTSEGMVGQLQARRAAGVILKMVQNGTIA
GRAVLVAGPPSTGKTALAMGVSQSLGKDVPFTAIAGSEIFSLELSKTEAL
TQAFRKSIGIKIKEETELIEGEVVEIQIDRSITGGHKQGKLTIKTTDMET
IYELGNKMIDGLTKEKVLAGDVISIDKASGKITKLGRSFARSRDYDAMGA
DTRFVQCPEGELQKRKTVVHTVSLHEIDVINSRTQGFLALFTGDTGEIRS
EVRDQINTKVAEWKEEGKAEIVPGVLFIDEVHMLDIECFSFINRALEDEF
APIVMMATNRGVSKTRGTNYKSPHGLPLDLLDRSIIITTKSYNEQEIKTI
LSIRAQEEEVELSSDALDLLTKTGVETSLRYSSNLISVAQQIAMKRKNNT
VEVEDVKRAYLLFLDSARSVKYVQENESQYIDDQGNVQISIA

3D structure

PDB

8eu9 Reorientation of INO80 on hexasomes reveals basis for mechanistic versatility.

Chain

Resolution

3.48 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

3.6.4.12: DNA helicase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	W	E139 E142 I143 Q144 I145 D146 R147 H153 Q155 K157 E170 K183 A186 F206 R208	E122 E125 I126 Q127 I128 D129 R130 H136 Q138 K140 E153 K166 A169 F189 R191
BS02	ADP	W	H23 H25 M44 V45 S78 T79 G80 K81 T82 Y359 I367 R397	H6 H8 M27 V28 S61 T62 G63 K64 T65 Y342 I350 R380

Gene Ontology

	Molecular Function
GO:0003678	DNA helicase activity
GO:0004386	helicase activity
GO:0005515	protein binding
GO:0005524	ATP binding
GO:0008094	ATP-dependent activity, acting on DNA
GO:0016787	hydrolase activity
GO:0016887	ATP hydrolysis activity
GO:0043138	3'-5' DNA helicase activity
GO:0043139	5'-3' DNA helicase activity

	Biological Process
GO:0000492	box C/D snoRNP assembly
GO:0006281	DNA repair
GO:0006325	chromatin organization
GO:0006338	chromatin remodeling
GO:0006355	regulation of DNA-templated transcription
GO:0006357	regulation of transcription by RNA polymerase II
GO:0006364	rRNA processing
GO:0032508	DNA duplex unwinding
GO:0050821	protein stabilization

	Cellular Component
GO:0000785	chromatin
GO:0000812	Swr1 complex
GO:0005634	nucleus
GO:0005654	nucleoplasm
GO:0031011	Ino80 complex
GO:0035267	NuA4 histone acetyltransferase complex
GO:0097255	R2TP complex

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:8eu9, PDBe:8eu9, PDBj:8eu9
PDBsum	8eu9
PubMed	37384669
UniProt	Q12464\|RUVB2_YEAST RuvB-like protein 2 (Gene Name=RVB2)

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