Structure of PDB 5wfs Chain W

Receptor sequence

>5wfsW (length=75) Species: 562 (Escherichia coli)

TRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTKFHAGANVGCGRDHTL
FAKADGKVKFEVKGPKNRKFISIEA

3D structure

• PDB: 5wfs Cryo-EM shows stages of initial codon selection on the ribosome by aa-tRNA in ternary complex with GTP and the GTPase-deficient EF-TuH84A.
• Chain: W
• Resolution: 3.0 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	W	T6 N8 R10 D11 S12 E13 A14 K15 R16 L17 K20 R21 F22 G23 E25 A29 G30 S31 I32 R35 R37 G38 T39 G50 R51 H53 F56 F65 K71 R73 K74	T1 N3 R5 D6 S7 E8 A9 K10 R11 L12 K15 R16 F17 G18 E20 A24 G25 S26 I27 R30 R32 G33 T34 G45 R46 H48 F51 F60 K66 R68 K69
BS02	rna	W	K68 G69 P70	K63 G64 P65

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome

External links

• PDB: RCSB:5wfs, PDBe:5wfs, PDBj:5wfs
• PDBsum: 5wfs
• PubMed: 29733411
• UniProt: P0A7L8|RL27_ECOLI Large ribosomal subunit protein bL27 (Gene Name=rpmA)