Structure of PDB 5wfk Chain W

Receptor sequence

>5wfkW (length=75) Species: 83333 (Escherichia coli K-12)

TRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTKFHAGANVGCGRDHTL
FAKADGKVKFEVKGPKNRKFISIEA

3D structure

• PDB: 5wfk Cryo-EM shows stages of initial codon selection on the ribosome by aa-tRNA in ternary complex with GTP and the GTPase-deficient EF-TuH84A.
• Chain: W
• Resolution: 3.4 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	W	N8 R10 S12 E13 A14 K15 R16 K20 F22 G23 E25 L28 A29 G30 S31 I32 R35 R37 G38 T39 F41 H42 G50 R51 D52 H53 F56 K58 F65 R73 K74	N3 R5 S7 E8 A9 K10 R11 K15 F17 G18 E20 L23 A24 G25 S26 I27 R30 R32 G33 T34 F36 H37 G45 R46 D47 H48 F51 K53 F60 R68 K69
BS02	rna	W	G69 P70	G64 P65

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome

External links

• PDB: RCSB:5wfk, PDBe:5wfk, PDBj:5wfk
• PDBsum: 5wfk
• PubMed: 29733411
• UniProt: P0A7L8|RL27_ECOLI Large ribosomal subunit protein bL27 (Gene Name=rpmA)