Structure of PDB 8gym Chain V1

Receptor sequence

>8gymV1 (length=442) Species: 312017 (Tetrahymena thermophila SB210)

RSYGNLKDQDRIFTNLYRDGDPFVKGALKRGDWHQTKEILSNGPEWIIDE
IKKSGLRGRGGAGFLSGLKYSFMPKVNPDGRPSYLVINSDESEPGTCKDR
EILRNDPHKLVEGALVVGFSMRARAAYIYIRGEFWVEANILQQAIDEAYA
KGFIGKNACGSGYDFDVYIHRGAGAYICGEETGLIESIEGKAGQPRVKPP
FPANAGLYGCPTTVTNVETVAVCPTIMRRGASWFASFGRPNNAGTKLYCI
SGHVNNPCTVEEEMSIPLRELLEKHCGGVRGGWDNLLAVIPGGSSVPMMP
KNVCDDVLMDFDALKAVGSGLGTAAVIVMDKSTDPIDAILRLSKFYKHES
CGQCTPCREGTGWIVDVMERLLVGNADYAEIDMLQQVTQQIEMHTICALG
DAAAWPVQGLIKNFREEIEDRIDSYHAKHPQLKKSRKSNPQI

3D structure

• PDB: 8gym Structures of Tetrahymena thermophila respiratory megacomplexes on the tubular mitochondrial cristae.
• Chain: V1
• Resolution: 2.96 Å

Enzymatic activity

• Enzyme Commision number: 7.1.1.2: NADH:ubiquinone reductase (H(+)-translocating).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SF4	V1	I206 P224 C380 G381 Q382 C383 C386 T424 C426 L428 G429	I177 P195 C351 G352 Q353 C354 C357 T395 C397 L399 G400
BS02	FMN	V1	G87 G89 N117 D119 Y205 E209 E210 T244 N245 T248 A427 L428	G58 G60 N88 D90 Y176 E180 E181 T215 N216 T219 A398 L399

Gene Ontology

Molecular Function

• GO:0008137 NADH dehydrogenase (ubiquinone) activity
• GO:0010181 FMN binding
• GO:0046872 metal ion binding
• GO:0051287 NAD binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Biological Process

• GO:0006120 mitochondrial electron transport, NADH to ubiquinone
• GO:1902600 proton transmembrane transport

Cellular Component

• GO:0005739 mitochondrion
• GO:0005743 mitochondrial inner membrane
• GO:0045271 respiratory chain complex I

External links

• PDB: RCSB:8gym, PDBe:8gym, PDBj:8gym
• PDBsum: 8gym
• PubMed: 37248254
• UniProt: Q23KE4