Structure of PDB 8esw Chain V1

Receptor sequence

>8eswV1 (length=439) Species: 7227 (Drosophila melanogaster)

PGTPPPQTKTKFGPLADEDRIFTNLYGRHDWRLKGALKRGDWYKTKEIVL
KGADWIVNEIKTSGLRGRGGAGFPSGMKWSFMNKPGDGRPKYLVVNADEG
EPGTCKDREIMRHDPHKLVEGCLIAGRAMGAQAAYIYIRGEFYNEASNMQ
LAIAEAYQAGLIGKNACGTGYDFDVFMHRGAGAYICGEETALIESLEGKQ
GKPRLKPPFPADVGVFGCPTTVTNVETVAVAPTICRRGGVWFASFGRTRN
SGTKLFNISGHVNRPCTVEEEMSIPLKELIERHCGGVTGGWDNLLGVIPG
GSSTPIIPKNVCDDVIMDFDGLIAAQTSLGTAAIIVMDKSTDVIKAIARL
ISFYKHESCGQCTPCREGIGWMNKIMTRFVKGDAQPAEIDMLWEISKQIE
GHTICALGDGAAWPVQGLIRHFRPEIEKRMQLHAKRVSN

3D structure

• PDB: 8esw Resting mitochondrial complex I from Drosophila melanogaster adopts a helix-locked state.
• Chain: V1
• Resolution: 3.3 Å

Enzymatic activity

• Enzyme Commision number: 7.1.1.2: NADH:ubiquinone reductase (H(+)-translocating).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	V1	Y178 N179 Q185 D209	Y143 N144 Q150 D174
BS02	FMN	V1	G102 G104 K113 N131 D133 E134 G222 E223 N259 A441	G67 G69 K78 N96 D98 E99 G187 E188 N224 A406
BS03	SF4	V1	I220 P238 C394 Q396 C397 C400 T438 I439 C440 L442	I185 P203 C359 Q361 C362 C365 T403 I404 C405 L407

Gene Ontology

Molecular Function

• GO:0008137 NADH dehydrogenase (ubiquinone) activity
• GO:0010181 FMN binding
• GO:0046872 metal ion binding
• GO:0051287 NAD binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Biological Process

• GO:0006120 mitochondrial electron transport, NADH to ubiquinone
• GO:1902600 proton transmembrane transport

Cellular Component

• GO:0005739 mitochondrion
• GO:0005743 mitochondrial inner membrane
• GO:0045271 respiratory chain complex I

External links

• PDB: RCSB:8esw, PDBe:8esw, PDBj:8esw
• PDBsum: 8esw
• PubMed: 36952377
• UniProt: Q9VMI3