Structure of PDB 7x3t Chain V

Receptor sequence

>7x3tV (length=792) Species: 559292 (Saccharomyces cerevisiae S288C)

DLEGTTKRFEHLLSLSGLFKHFIESKADPKFRQVLDVLEENQFRESPAYV
NGQLRPYQIQGVNWLVSLHKNKIAGILADEMGLGKTLQTISFLGYLRYIE
KIPGPFLVIAPKSTLNNWLREINRWTPDVNAFILQGDKEERAELIQKKLL
GCDFDVVIASYEIIIREKSPLKKINWEYIIIDEAHRIKNEESMLSQVLRE
FTSRNRLLITGTPLQNNLHELWALLNFLLPDIFSDAQDFDDWFSSESTEE
DQDKIVKQLHTVLQPFLLRRIKSDVETSLLPKKELNLYVGMSSMQKKWYK
KILEKDESKTRLLNIMMQLRKCCNHPYLFDGAEPGPPYTTDEHLVYNAAK
LQVLDKLLKKLKEEGSRVLIFSQMSRLLDILEDYCYFRNYEYCRIDGSTA
HEDRIQAIDDYNAPDSKKFVFLLTTRAGGLGINLTSADVVVLYDSDWNPQ
ADLQAMDRAHRIGQKKQVKVFRLVTDNSVEEKILERATQKLRLDQLVIQQ
NRTSNPTKRERHPRMPKPHVFHSHQLQPPQLKVLYEKERMWTAKKTGYVP
TMDDVKAAYGDISDEEEKKQKLELLKLSVNNSQPLTEEEEKMKADWESEG
FTNWNKLEFRKFITVSGKYGRNSIQAIARELAPGKTLEEVRAYAKAFWSN
IERIEDYEKYLKIIENEEEKIKRVKMQQEALRRKLSEYKNPFFDLKLKHP
PSSNNKRTYSEEEDRFILLMLFKYGLDRDDVYELVRDEIRDCPLFELDFY
FRSRTPVELARRGNTLLQCLEKEFNAGIVLDDATKDRMKKED

3D structure

• PDB: 7x3t ISW1a-dinucleosome
• Chain: V
• Resolution: 5.4 Å

Enzymatic activity

• Enzyme Commision number: 3.6.4.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	V	N331 S334 M335 M469 W600 N601 K643 N987	N189 S192 M193 M316 W447 N448 K490 N704
BS02	dna	V	K254 K280 E304 R308 L466 K474 M527 S528 G550 S551 R557 T577 R579 A580	K112 K138 E162 R166 L313 K321 M374 S375 G397 S398 R404 T424 R426 A427
BS03	ADP	V	Q195 R197 Q200 G224 G226 K227 T228 L229 E263 W267 R614 I615	Q53 R55 Q58 G82 G84 K85 T86 L87 E121 W125 R461 I462
BS04	BEF	V	M223 G224 K227 G584 R614	M81 G82 K85 G431 R461
BS05	MG	V	G226 K227 T228	G84 K85 T86

Gene Ontology

Molecular Function

• GO:0000182 rDNA binding
• GO:0000976 transcription cis-regulatory region binding
• GO:0003677 DNA binding
• GO:0003730 mRNA 3'-UTR binding
• GO:0004386 helicase activity
• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0008094 ATP-dependent activity, acting on DNA
• GO:0016787 hydrolase activity
• GO:0016887 ATP hydrolysis activity
• GO:0031491 nucleosome binding
• GO:0140658 ATP-dependent chromatin remodeler activity

Biological Process

• GO:0001178 regulation of transcriptional start site selection at RNA polymerase II promoter
• GO:0006325 chromatin organization
• GO:0006338 chromatin remodeling
• GO:0006354 DNA-templated transcription elongation
• GO:0006355 regulation of DNA-templated transcription
• GO:0006363 termination of RNA polymerase I transcription
• GO:0006369 termination of RNA polymerase II transcription
• GO:0007062 sister chromatid cohesion
• GO:0009408 response to heat
• GO:0045944 positive regulation of transcription by RNA polymerase II
• GO:0046832 negative regulation of RNA export from nucleus
• GO:1902275 regulation of chromatin organization
• GO:1903895 negative regulation of IRE1-mediated unfolded protein response

Cellular Component

• GO:0005634 nucleus
• GO:0016587 Isw1 complex
• GO:0030874 nucleolar chromatin
• GO:0036436 Isw1a complex
• GO:0036437 Isw1b complex

External links

• PDB: RCSB:7x3t, PDBe:7x3t, PDBj:7x3t
• PDBsum: 7x3t
• PubMed: 38177688
• UniProt: P38144|ISW1_YEAST ISWI chromatin-remodeling complex ATPase ISW1 (Gene Name=ISW1)