Structure of PDB 5fg9 Chain V

Receptor sequence

>5fg9V (length=230) Species: 559292 (Saccharomyces cerevisiae S288C)

TSVGTTIVGVKFNNGVVIAADTRSTQGPIVADKNCAKLHRISPKIWCAGA
GTAADTEAVTQLIGSNIELHSLYTSREPRVVSALQMLKQHLFKYQGHIGA
YLIVAGVDPTGSHLFSIHAHGSTDVGYYLSLGSGSLAAMAVLESHWKQDL
TKEEAIKLASDAIQAGIWNDLGSGSNVDVCVMEIGKDAEYLRNYLTPNVR
EEKQKSYKFPRGTTAVLKESIVNICDIQEE

3D structure

• PDB: 5fg9 A unified mechanism for proteolysis and autocatalytic activation in the 20S proteasome.
• Chain: V
• Resolution: 2.6 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): T1 D17 R19 K33 G47 S129 D166 S169
• Catalytic site (residue number reindexed from 1): T5 D21 R23 K37 G51 S133 D170 S173
• Enzyme Commision number: 3.4.25.1: proteasome endopeptidase complex.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MG	V	I163 D166 S169	I167 D170 S173

Gene Ontology

Molecular Function

• GO:0004175 endopeptidase activity
• GO:0004298 threonine-type endopeptidase activity
• GO:0005515 protein binding

Biological Process

• GO:0006508 proteolysis
• GO:0010499 proteasomal ubiquitin-independent protein catabolic process
• GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process
• GO:0051603 proteolysis involved in protein catabolic process

Cellular Component

• GO:0000502 proteasome complex
• GO:0005634 nucleus
• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005839 proteasome core complex
• GO:0019774 proteasome core complex, beta-subunit complex
• GO:0034515 proteasome storage granule

External links

• PDB: RCSB:5fg9, PDBe:5fg9, PDBj:5fg9
• PDBsum: 5fg9
• PubMed: 26964885
• UniProt: P25043|PSB2_YEAST Proteasome subunit beta type-2 (Gene Name=PUP1)