Structure of PDB 5fg7 Chain V

Receptor sequence

>5fg7V (length=228) Species: 559292 (Saccharomyces cerevisiae S288C)

KATSTGATIVGVKFNNGVVIAADTRSTQGPIVADKNCAKLHRISPKIWCA
GAGTAADTEAVTQLIGSNIELHSLYTSREPRVVSALQMLKQHLFKYQGHI
GAYLIVAGVDPTGSHLFSIHAHGSTDVGYYLSLGSGSLAAMAVLESHWKQ
DLTKEEAIKLASDAIQAGIWNDLGSGSNVDVCVMEIGKDAEYLRNYLTPN
VREEKQKSYKFPRGTTAVLKESIVNICD

3D structure

• PDB: 5fg7 A unified mechanism for proteolysis and autocatalytic activation in the 20S proteasome.
• Chain: V
• Resolution: 2.7 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): A1 D17 R19 K33 G47 S129 D166 S169
• Catalytic site (residue number reindexed from 1): A7 D23 R25 K39 G53 S135 D172 S175
• Enzyme Commision number: 3.4.25.1: proteasome endopeptidase complex.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MG	V	I163 D166 S169	I169 D172 S175

Gene Ontology

Molecular Function

• GO:0004175 endopeptidase activity
• GO:0004298 threonine-type endopeptidase activity
• GO:0005515 protein binding

Biological Process

• GO:0006508 proteolysis
• GO:0010499 proteasomal ubiquitin-independent protein catabolic process
• GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process
• GO:0051603 proteolysis involved in protein catabolic process

Cellular Component

• GO:0000502 proteasome complex
• GO:0005634 nucleus
• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005839 proteasome core complex
• GO:0019774 proteasome core complex, beta-subunit complex
• GO:0034515 proteasome storage granule

External links

• PDB: RCSB:5fg7, PDBe:5fg7, PDBj:5fg7
• PDBsum: 5fg7
• PubMed: 26964885
• UniProt: P25043|PSB2_YEAST Proteasome subunit beta type-2 (Gene Name=PUP1)