Structure of PDB 6zqb Chain UX

Receptor sequence

>6zqbUX (length=174) Species: 559292 (Saccharomyces cerevisiae S288C)

TRKFGLVKRTLNTKKDQRLKKNQENTRNIPQVSSALFFQYNQAIKPPYQV
LIDTNFINFSIQKKVDIVRGMMDCLLAKCNPLITDCVMAELEKLGPKYRI
ALKLARDPRIKRLSCSHKGTYADDCLVHRVLQHKCYIVATNDAGLKQRIR
KIPGIPLMSVGGHAYVIEKLPDVF

3D structure

• PDB: 6zqb 90 S pre-ribosome transformation into the primordial 40 S subunit.
• Chain: UX
• Resolution: 3.9 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	UX	V13 K21	V7 K15
BS02	rna	UX	R42 Q162 R163	R27 Q147 R148
BS03	rna	UX	F10 Q23 K133 Y136	F4 Q17 K118 Y121
BS04	ZN	UX	C101 C130 H132 C140	C86 C115 H117 C125

Gene Ontology

Molecular Function

• GO:0004521 RNA endonuclease activity
• GO:0004540 RNA nuclease activity
• GO:0005515 protein binding
• GO:0008270 zinc ion binding

Biological Process

• GO:0000447 endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0000472 endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0000480 endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0006364 rRNA processing
• GO:0030490 maturation of SSU-rRNA
• GO:0042254 ribosome biogenesis

Cellular Component

• GO:0005634 nucleus
• GO:0005654 nucleoplasm
• GO:0005730 nucleolus
• GO:0032040 small-subunit processome

External links

• PDB: RCSB:6zqb, PDBe:6zqb, PDBj:6zqb
• PDBsum: 6zqb
• PubMed: 32943521
• UniProt: Q05498|FCF1_YEAST rRNA-processing protein FCF1 (Gene Name=FCF1)