Structure of PDB 7alp Chain U

Receptor sequence

>7alpU (length=1935) Species: 1173018 (Phlebovirus WCH/97/HN/China/2011)

MNLEVLCGRINVENGLSLGEPGLYDQIYDRPGLPDLDVTVDATGVTVDLI
TIQLSEAYKINHDFTFSGLSKTTDRRLSEVFPITHDGSDGMTPDVIHTRL
DGTIVVVEFTTTRSHNIGGLEAAYRTKIEKYRDPISRRVDIMENPRVFFG
VIVVSSGGVLSNMPLTQDEAEELMYRFCIANEIYTKARQKSEEELEAISR
ALSFFSLFEPNIERVEGTFPNSEIEMLEQFLSTPADVDFITKTLKAKEVE
AYADLCDSHYLKPEKTIQERLEINRCEAIDKTQDLLAGLHARSNKQTSLN
RGTVKLPPWLPKPSSESIDIKTDSGFGSLMDHGAYGELWAKCLLDVSLGN
VEGVVSDPAKELDIKEAKITYRRFKPALSSSARQEFSLQGDALSPFLDVE
DIGDFLTFNNLLADSRYGDESVQRAVSILLEKASAMQDTELTHALNDSFK
RNLSSNVVQWSLWVSCLAQELASALKQHCRAGEFIIKKLKFWPIYVIIKP
TKSSSHIFYSLGIRKADVTRRLTGRVFSETIDAGEWELTEFKSLKTCKLT
NLVNLPCTMLNSIAFWREKLGVAPWLVRKPCSELREQVGLTFLISLEDKS
KTEEIITLTRYTQMEGFVSPPMLPKPQKMLGKLDGPLRTKLQVYLLRKHL
DCMVRIASQPFSLIPREGRVEWGGTFHAISGRSTNLENMVNSWYIGYYKN
KEESTELNALGEMYKKIVEMEEDKPSSPEFLGWGDTDSPKKHEFSRSFLR
AACSSLEREIAQRHGRQWKQNLEERVLREIGTKNILDLASMKATSNFSKD
WELYSEVQTKEYHRSKLLEKMATLIEKGVMWYIDAVGQAWKAVLDDGCMR
ICLFKKNQHGGLREIYVMDANARLVQFGVETMARCVCELSPHETVANPRL
KNSIIENHGLKSARSLGPGSININSSNDAKKWNQGHYTTKLALVLCWFMP
AKFHRFIWAAISMFRRKKMMVDLRFLAHLSSPFREAMTDAFHGNREVSWM
DKGRTYIKTETGMMQGILHFTSSLLHSCVQSFYKSYFVSKLKEGYMGESI
SGVVDVIEGSDDSAIMISIRPKSDMDEVRSRFFVANLLHSVKFLNPLFGI
YSSEKSTVNTVYCVEYNSEFHFHRHLVRPTLRWIAASHQISETEALASRQ
EDYSNLLTQCLEGGASFSLTYLIQCAQLLHHYMLLGLCLHPLFGTFMGML
ISDPDPALGFFLMDNPAFAGGAGFRFNLWRACKTTDLGRKYAYYFNEIQG
KTKGDEDYRALDATSGGTLSHSVMVYWGDRKKYQALLNRMGLPEDWVEQI
DENPGVLYRRAANKKELLLKLAEKVHSPGVTSSLSKGHVVPRVVAAGVYL
LSRHCFRFSSTQKASLIKLLMMSSLNPNQERMLFPQAQEYDRVCTLLEEV
EHLTGKFVVRERNIVRSRIDLFQEPVDLRCKAEDLVSEVWFGLKRTKLGP
RLLKEEWDKLRASFAWLSTDPSETLRDGPFLSHVQFRNFIAHVDAKSRSV
RLLGAPVVTTISQVVRMNFFPGFSLEALESISILKHVLFMVLNGPYTEEY
KLEMIIEAFSTLVIPQPSEVIRKSRTMTLCLLSNSILDQIERAQSGTLGG
FSKPQKTFIRPGGGVGYKGKGVWTGVMEDTHVQILIDGDGTSNWLEEIRL
SSDARLYDVIESIRRLCDDLGINNRVASAYRGHCMVRLSGFKIKPASRTD
GCPVRIMERGFRIRELQNPDEVKMRVRGDILNLSVTIQEGRVMNILSYRP
RDTDISESAAAYLWSNRDLFSFPSCSWICLKASVLLANDRKTQGIDNRAM
GNIFRDCLEGSLRKQGLMRSKLTEMVEKNVVPLTTQELVDILEEDIDFSD
VIAVELSEGSLDIESIFDGAPILWSAEVEEFGEGVTLMDQAAITMCAIMG
KEGCRGLLTEKRCMAAIREQVRPFEDSISWVSDQF

3D structure

• PDB: 7alp Errors in the deposited SFTSV L protein structure.
• Chain: U
• Resolution: 3.4 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.
  3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MG	U	D1127 E1180	D1062 E1115

Gene Ontology

Molecular Function

• GO:0003968 RNA-dependent RNA polymerase activity
• GO:0016740 transferase activity
• GO:0016787 hydrolase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0001172 RNA-templated transcription
• GO:0006351 DNA-templated transcription
• GO:0019079 viral genome replication
• GO:0039694 viral RNA genome replication

Cellular Component

• GO:0044165 host cell endoplasmic reticulum
• GO:0044172 host cell endoplasmic reticulum-Golgi intermediate compartment
• GO:0044177 host cell Golgi apparatus
• GO:0044423 virion component

External links

• PDB: RCSB:7alp, PDBe:7alp, PDBj:7alp
• PDBsum: 7alp
• PubMed: 33927385
• UniProt: I0DF35|L_SFTS RNA-directed RNA polymerase L