Structure of PDB 5lry Chain T

Receptor sequence
>5lryT (length=263) Species: 199310 (Escherichia coli CFT073) [Search protein sequence]
PRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGT
QAEEVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAA
GASAIIAWGTCASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPD
VMSAIITYMVTFDRLPDVDRMGRPLMFYGQRIHDKCYRRAHFDAGEFVQS
WDDDAARKGYCLYKMGCKGPTTYNACSSTRWNDGVSFPIQSGHGCLGCAE
NGFWDRGSFYSRV
3D structure
PDB5lry Mechanistic Exploitation of a Self-Repairing, Blocked Proton Transfer Pathway in an O2-Tolerant [NiFe]-Hydrogenase.
ChainT
Resolution1.4 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) C149 C249 C252
Catalytic site (residue number reindexed from 1) C145 C245 C248
Enzyme Commision number 1.12.99.6: hydrogenase (acceptor).
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 SF4 T H187 C190 R193 C215 L216 Y217 C221 I243 H183 C186 R189 C211 L212 Y213 C217 I239
BS02 F3S T N228 C230 W235 P242 C249 L250 C252 N224 C226 W231 P238 C245 L246 C248
BS03 SF3 T C17 C19 C20 E76 C115 C120 C149 C13 C15 C16 E72 C111 C116 C145
Gene Ontology
Molecular Function
GO:0005515 protein binding
GO:0008901 ferredoxin hydrogenase activity
GO:0009055 electron transfer activity
GO:0016491 oxidoreductase activity
GO:0033748 hydrogenase (acceptor) activity
GO:0046872 metal ion binding
GO:0051536 iron-sulfur cluster binding
GO:0051538 3 iron, 4 sulfur cluster binding
GO:0051539 4 iron, 4 sulfur cluster binding
Biological Process
GO:0006113 fermentation
GO:0009061 anaerobic respiration
GO:0009267 cellular response to starvation
GO:0019645 anaerobic electron transport chain
GO:1902421 hydrogen metabolic process
Cellular Component
GO:0005886 plasma membrane
GO:0009375 ferredoxin hydrogenase complex
GO:0016020 membrane
GO:0044569 [Ni-Fe] hydrogenase complex
GO:0098567 periplasmic side of plasma membrane

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:5lry, PDBe:5lry, PDBj:5lry
PDBsum5lry
PubMed30070475
UniProtP69739|MBHS_ECOLI Hydrogenase-1 small chain (Gene Name=hyaA)

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