Structure of PDB 3use Chain T

Receptor sequence

>3useT (length=268) Species: 562 (Escherichia coli) [Search protein sequence]

KPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAG
TQAEEVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAA
AGASAIIAWGTCASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIP
DVMSAIITYMVTFDRLPDVDRMGRPLMFYGQRIHDKCYRRAHFDAGEFVQ
SWDDDAARKGYCLYKMGCKGPTTYNACSSTRWNDGVSFPIQSGHGCLGCA
ENGFWDRGSFYSRVVDIP

3D structure

PDB

3use X-ray crystallographic and computational studies of the O2-tolerant [NiFe]-hydrogenase 1 from Escherichia coli.

Chain

Resolution

1.67 Å

3D
structure

Catalytic site residues are labeled in the structure
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Enzymatic activity

Catalytic site (original residue number in PDB)	C149 C249 C252
Catalytic site (residue number reindexed from 1)	C146 C246 C249
Enzyme Commision number	1.12.99.6: hydrogenase (acceptor).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	SF4	T	H187 C190 R192 R193 C215 L216 Y217 C221 P224 I243	H184 C187 R189 R190 C212 L213 Y214 C218 P221 I240
BS02	F3S	T	N228 C230 W235 P242 C249 L250 C252	N225 C227 W232 P239 C246 L247 C249
BS03	F4S	T	C17 C19 C20 C115 C120 C149	C14 C16 C17 C112 C117 C146
BS04	SF3	T	C17 T18 C19 C20 E76 C115 C120 C149	C14 T15 C16 C17 E73 C112 C117 C146

Gene Ontology

	Molecular Function
GO:0005515	protein binding
GO:0008901	ferredoxin hydrogenase activity
GO:0009055	electron transfer activity
GO:0016491	oxidoreductase activity
GO:0033748	hydrogenase (acceptor) activity
GO:0046872	metal ion binding
GO:0051536	iron-sulfur cluster binding
GO:0051538	3 iron, 4 sulfur cluster binding
GO:0051539	4 iron, 4 sulfur cluster binding

	Biological Process
GO:0006113	fermentation
GO:0009061	anaerobic respiration
GO:0009267	cellular response to starvation
GO:0019645	anaerobic electron transport chain
GO:1902421	hydrogen metabolic process

	Cellular Component
GO:0005886	plasma membrane
GO:0009375	ferredoxin hydrogenase complex
GO:0016020	membrane
GO:0044569	[Ni-Fe] hydrogenase complex
GO:0098567	periplasmic side of plasma membrane

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Molecular Function

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Biological Process

View graph for
Cellular Component

External links

PDB	RCSB:3use, PDBe:3use, PDBj:3use
PDBsum	3use
PubMed	22431599
UniProt	P69739\|MBHS_ECOLI Hydrogenase-1 small chain (Gene Name=hyaA)

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