Structure of PDB 8k82 Chain SI

Receptor sequence

>8k82SI (length=188) Species: 4932 (Saccharomyces cerevisiae)

GISRDSRHKRSATGAKRAQFRKKRKFELGRQPANTKIGAKRIHSVRTRGG
NKKYRALRIETGNFSWASEGISKKTRIAGVVYHPSNNELVRTNTLTKAAI
VQIDATPFRQWFEAHYGQTLGKKSKNAERKWAARAASAKIESSVESQFSA
GRLYACISSRPGQSGRCDGYILEGEELAFYLRRLTAKK

3D structure

• PDB: 8k82 Structural basis for differential inhibition of eukaryotic ribosomes by tigecycline.
• Chain: SI
• Resolution: 3.0 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	SI	G2 R5 D6 R8 H9 K10 R11 S12 A13 T14 G15 A16 K17 F21 R22 K23 K24 R25 K26 F27 L29 G30 R31 Q32 P33 K41 R42 H44 R47 T48 R49 G50 G51 K54 R56 L58 N64 I72 S73 K75 S86 T97 K98 S136 K137 N138 R141 K142 R146 S170 S171 R172 G174 Q175 S176 R178 D180	G1 R4 D5 R7 H8 K9 R10 S11 A12 T13 G14 A15 K16 F20 R21 K22 K23 R24 K25 F26 L28 G29 R30 Q31 P32 K40 R41 H43 R46 T47 R48 G49 G50 K53 R55 L57 N63 I71 S72 K74 S85 T96 K97 S124 K125 N126 R129 K130 R134 S158 S159 R160 G162 Q163 S164 R166 D168
BS02	rna	SI	R77 E89 R92 A162 G163 R164 K200	R76 E88 R91 A150 G151 R152 K188

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0000462 maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005840 ribosome
• GO:0022627 cytosolic small ribosomal subunit
• GO:0030686 90S preribosome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:8k82, PDBe:8k82, PDBj:8k82
• PDBsum: 8k82
• PubMed: 38942792
• UniProt: P0CX39|RS8A_YEAST Small ribosomal subunit protein eS8A (Gene Name=RPS8A)