Structure of PDB 3ja1 Chain S3

Receptor sequence

>3ja1S3 (length=702) Species: 562 (Escherichia coli)

ARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVHDGAATMD
WMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGAVDFTIEVER
SMRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANF
LKVVNQIKTRLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVT
FEYEDIPADMVELANEWHQNLIESAAEASEELMEKYLGGEELTEAEIKGA
LRQRVLNNEIILVTCGSAFKNKGVQAMLDAVIDYLPSPVDVPAINGILDD
GKDTPAERHASDDEPFSALAFKIATDPFVGNLTFFRVYSGVVNSGDTVLN
SVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVTTGDTLCDPD
APIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTDE
ESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDV
EGKHAKQSGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIP
AVDKGIQEQLKAGPLAGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAF
KEGFKKAKPVLLEPIMKVEVETPEENTGDVIGDLSRRRGMLKGQESEVTG
VKIHAEVPLSEMFGYATQLRSLTKGRASYTMEFLKYDEAPSNVAQAVIEA
RG

3D structure

• PDB: 3ja1 Activation of GTP hydrolysis in mRNA-tRNA translocation by elongation factor G.
• Chain: S3
• Resolution: 3.6 Å

Enzymatic activity

• Enzyme Commision number: 3.6.5.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	S3	F328 V329 R356 R358 G360 R511	F328 V329 R356 R358 G360 R511
BS02	rna	S3	I18 R58 Q119 G628 R636 L641 K642 G643 Q644 R670 S671 K674 G675	I18 R58 Q119 G628 R636 L641 K642 G643 Q644 R670 S671 K674 G675
BS03	GTP	S3	D19 A20 G21 K22 T23 T24 I60 T61 N141 K142 D144 S267 F269	D19 A20 G21 K22 T23 T24 I60 T61 N141 K142 D144 S267 F269

Gene Ontology

Molecular Function

• GO:0003746 translation elongation factor activity
• GO:0003924 GTPase activity
• GO:0005525 GTP binding
• GO:0016787 hydrolase activity
• GO:0097216 guanosine tetraphosphate binding

Biological Process

• GO:0006412 translation
• GO:0006414 translational elongation
• GO:0032790 ribosome disassembly

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:3ja1, PDBe:3ja1, PDBj:3ja1
• PDBsum: 3ja1
• PubMed: 26229983
• UniProt: P0A6M8|EFG_ECOLI Elongation factor G (Gene Name=fusA)