Structure of PDB 8pov Chain S

Receptor sequence

>8povS (length=269) Species: 381666 (Cupriavidus necator H16)

PRTPVLWLHGLECTGCSESFIRSAHPLAKDVVLSMISLDYDDTLMAAAGH
QAEAILEEIMTKYKGNYILAVEGNPPLNQDGMSCIIGGRPFIEQLKYVAK
DAKAIISWGSCASWGGVQAAKPNPTQATPVHKVITDKPIIKVPGCPPIAE
VMTGVITYMLTFDRIPELDRQGRPKMFYSQRIHDKCYRRPHFDAGQFVEE
WDDESARKGFCLYKMGCKGPTTYNACSTTRWNEGTSFPIQSGHGCIGCSE
DGFWDKGSFYDRLTGISQF

3D structure

• PDB: 8pov Stepwise conversion of the Cys 6 [4Fe-3S] to a Cys 4 [4Fe-4S] cluster and its impact on the oxygen tolerance of [NiFe]-hydrogenase.
• Chain: S
• Resolution: 1.92 Å

Enzymatic activity

• Enzyme Commision number: 1.12.99.6: hydrogenase (acceptor).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SF4	S	H187 C190 R192 R193 C215 L216 Y217 C221 I243	H183 C186 R188 R189 C211 L212 Y213 C217 I239
BS02	F3S	S	N228 C230 W235 P242 C249 I250 C252	N224 C226 W231 P238 C245 I246 C248
BS03	SF4	S	C17 G19 C20 G113 C115 C149 P150	C13 G15 C16 G109 C111 C145 P146

Gene Ontology

Molecular Function

• GO:0005515 protein binding
• GO:0008901 ferredoxin hydrogenase activity
• GO:0009055 electron transfer activity
• GO:0016491 oxidoreductase activity
• GO:0033748 hydrogenase (acceptor) activity
• GO:0046872 metal ion binding
• GO:0051536 iron-sulfur cluster binding
• GO:0051538 3 iron, 4 sulfur cluster binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Biological Process

• GO:0009061 anaerobic respiration

Cellular Component

• GO:0005886 plasma membrane
• GO:0009375 ferredoxin hydrogenase complex
• GO:0016020 membrane
• GO:0044569 [Ni-Fe] hydrogenase complex

External links

• PDB: RCSB:8pov, PDBe:8pov, PDBj:8pov
• PDBsum: 8pov
• PubMed: 37860641
• UniProt: P31892|MBHS_CUPNH Uptake hydrogenase small subunit (Gene Name=hoxK)