Structure of PDB 5wfk Chain S

Receptor sequence

>5wfkS (length=109) Species: 83333 (Escherichia coli K-12) [Search protein sequence]

METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAAVLVKKV
LESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRT
SHITVVVSD

3D structure

PDB

5wfk Cryo-EM shows stages of initial codon selection on the ribosome by aa-tRNA in ternary complex with GTP and the GTPase-deficient EF-TuH84A.

Chain

Resolution

3.4 Å

3D
structure

Enzymatic activity

Enzyme Commision number

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	S	I4 K6 H7 R8 H9 R11 S13 Q15 K16 R18 N40 K41 K42 K49 S53 A56 N57 H60 N61 F75 D77 E78 P80 K83 R84 P87 R88 A89 K90 G91 R92 A93 D94 I96 L97 K98 R99 H102	I4 K6 H7 R8 H9 R11 S13 Q15 K16 R18 N40 K41 K42 K49 S53 A56 N57 H60 N61 F75 D77 E78 P80 K83 R84 P87 R88 A89 K90 G91 R92 A93 D94 I96 L97 K98 R99 H102

Gene Ontology

	Molecular Function
GO:0003735	structural constituent of ribosome
GO:0005515	protein binding
GO:0019843	rRNA binding
GO:0070180	large ribosomal subunit rRNA binding

	Biological Process
GO:0002181	cytoplasmic translation
GO:0006412	translation
GO:0042255	ribosome assembly
GO:0042256	cytosolic ribosome assembly
GO:0046677	response to antibiotic
GO:1902626	assembly of large subunit precursor of preribosome

	Cellular Component
GO:0005737	cytoplasm
GO:0005829	cytosol
GO:0005840	ribosome
GO:0015934	large ribosomal subunit
GO:0022625	cytosolic large ribosomal subunit
GO:1990904	ribonucleoprotein complex

External links

PDB	RCSB:5wfk, PDBe:5wfk, PDBj:5wfk
PDBsum	5wfk
PubMed	29733411
UniProt	P61175\|RL22_ECOLI Large ribosomal subunit protein uL22 (Gene Name=rplV)