Structure of PDB 5jrd Chain S

Receptor sequence
>5jrdS (length=264) Species: 199310 (Escherichia coli CFT073) [Search protein sequence]
KPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAG
TQAEEVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAA
AGASAIIAWGTCASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIP
DVMSAIITYMVTFDRLPDVDRMGRPLMFYGQRIHDKCYRRAHFDAGEFVQ
SWDDDAARKGYCLYKMGCKGPTTYNACSSTRWNDGVSFPIQSGHGCLGCA
ENGFWDRGSFYSRV
3D structure
PDB5jrd Importance of the Active Site "Canopy" Residues in an O2-Tolerant [NiFe]-Hydrogenase.
ChainS
Resolution1.2 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) C149 C249 C252
Catalytic site (residue number reindexed from 1) C146 C246 C249
Enzyme Commision number 1.12.99.6: hydrogenase (acceptor).
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 SF4 S H187 C190 R193 C215 L216 C221 I243 H184 C187 R190 C212 L213 C218 I240
BS02 F3S S N228 C230 W235 P242 C249 L250 C252 N225 C227 W232 P239 C246 L247 C249
BS03 SF3 S C17 T18 C19 C20 C115 C120 C149 C14 T15 C16 C17 C112 C117 C146
Gene Ontology
Molecular Function
GO:0008901 ferredoxin hydrogenase activity
GO:0009055 electron transfer activity
GO:0016491 oxidoreductase activity
GO:0033748 hydrogenase (acceptor) activity
GO:0046872 metal ion binding
GO:0051536 iron-sulfur cluster binding
GO:0051538 3 iron, 4 sulfur cluster binding
GO:0051539 4 iron, 4 sulfur cluster binding
Biological Process
GO:0009061 anaerobic respiration
Cellular Component
GO:0005886 plasma membrane
GO:0009375 ferredoxin hydrogenase complex
GO:0016020 membrane
GO:0044569 [Ni-Fe] hydrogenase complex

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Cellular Component
External links
PDB RCSB:5jrd, PDBe:5jrd, PDBj:5jrd
PDBsum5jrd
PubMed28001048
UniProtP69740|MBHS_ECOL6 Hydrogenase-1 small chain (Gene Name=hyaA)

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