Structure of PDB 4ttt Chain S

Receptor sequence
>4tttS (length=269) Species: 381666 (Cupriavidus necator H16) [Search protein sequence]
PRTPVLWLHGLECTCCSESFIRSAHPLAKDVVLSMISLDYDDTLMAAAGH
QAEAILEEIMTKYKGNYILAVEGNPPLNQDGMSCIIGGRPFIEQLKYVAK
DAKAIISWGSCASWGCVQAAKPNPTQATPVHKVITDKPIIKVPGCPPIAE
VMTGVITYMLTFDRIPELDRQGRPKMFYSQRIHDKCYRRPHFDAGQFVEE
WDDESARKGFCLYKMGCKGPTTYNACSTTRWNEGTSFPIQSGHGCIGCSE
DGFWDKGSFYDRLTGISQF
3D structure
PDB4ttt Tracking the route of molecular oxygen in O2-tolerant membrane-bound [NiFe] hydrogenase
ChainS
Resolution1.72 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) C149 P242 C249 C252
Catalytic site (residue number reindexed from 1) C145 P238 C245 C248
Enzyme Commision number 1.12.99.6: hydrogenase (acceptor).
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 SF4 S H187 C190 R192 R193 C215 L216 Y217 C221 P224 I243 H183 C186 R188 R189 C211 L212 Y213 C217 P220 I239
BS02 F3S S N228 C230 W235 P242 C249 I250 C252 N224 C226 W231 P238 C245 I246 C248
BS03 F4S S C17 T18 C19 C20 E76 S114 C115 C120 C149 C13 T14 C15 C16 E72 S110 C111 C116 C145
Gene Ontology
Molecular Function
GO:0005515 protein binding
GO:0008901 ferredoxin hydrogenase activity
GO:0009055 electron transfer activity
GO:0016491 oxidoreductase activity
GO:0033748 hydrogenase (acceptor) activity
GO:0046872 metal ion binding
GO:0051536 iron-sulfur cluster binding
GO:0051538 3 iron, 4 sulfur cluster binding
GO:0051539 4 iron, 4 sulfur cluster binding
Biological Process
GO:0009061 anaerobic respiration
Cellular Component
GO:0005886 plasma membrane
GO:0009375 ferredoxin hydrogenase complex
GO:0016020 membrane
GO:0044569 [Ni-Fe] hydrogenase complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:4ttt, PDBe:4ttt, PDBj:4ttt
PDBsum4ttt
PubMed
UniProtP31892|MBHS_CUPNH Uptake hydrogenase small subunit (Gene Name=hoxK)

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