Structure of PDB 6lqr Chain RE

Receptor sequence

>6lqrRE (length=1079) Species: 559292 (Saccharomyces cerevisiae S288C)

SNIFKLQIDELLEQVKLKQKHVLKVEKFLHKLYDILQEIPDWEEKSLAEV
DSFFKNKIVSVPFVDPKPIPQNTNYKFNYKKPDISLIGSFALKAGIYQPN
GSSIDTLLTMPKELFEKKDFLNFRCLHKRSVYLAYLTHHLLILLKKDKLD
SFLQLEYSYFDNDPLLPILRISCSKDYNFYKTRFSINLLIGFPYKVFEPK
KLLPNRNCIRILPATPLYNFSVLSSSTHENYLKYLYKTKKQTESFVEATV
LGRLWLQQRGFSSNMSHSGSLGGFGTFEFTILMAALLNGGGINSNKILLH
GFSSYQLFKGVIKYLATMDLCHDGHLQFHSNPASKYIDEGFQTPTLFDKS
TKVNILTKMTVSSYQILKEYAGETLRMLNNVVQDQFSNIFLTNISRFDNL
KYDLCYDVQLPLGKYNNLETSLAATFGSMERVKFITLENFLAHKITNVAR
YALGDRIKYIQIEMVGQKSDFPITKRKVYSNTGGNHFNFDFVRVKLIVNP
SECDKLVTKGPAHSETMSTEAAVFKNFWGIKSSLRRFKDGSITHCCVWST
SSSEPIISSIVNFALQKHVSKKAQISNETIKKFHNFLPLPNLPSSAKTSV
LNLSSFFNLKKSFDDLYKIIFQMKLPLSVKSILPVGSAFRYTSLCQPVPF
AYSDPDFFQDVILEFETSPKWPDEITSLEKAKTAFLLKIQEELSANSSTY
RSFFSRDESIPYNLEIVTLNILTPEGYGFKFRVLTERDEILYLRAIANAR
NELKPELEATFLKFTAKYLASVRHTRTLENISHSYQFYSPVVRLFKRWLD
THLLLGHITDELAELIAIKPFVDPAPYFIPGSLENGFLKVLKFISQWNWK
DDPLILDLVKPEERLTLAQYKGIQMNFTNLRNSDPNGTHLQFFVASKNDP
SGILYSSGIPLPIATRLTALAKVAVNLLQTHGLNQQTINLLFTPGLKDYD
FVVDLRTPIGLKSSCGILSAPSNFPENLNDLSEKMDPTYQLVKYLNLKYK
NSLILSSRKYIGVNGGEKGDKNVITGLIKPLFKGAHKFRVNLDCNVKPVD
DENVILNKEAIFHEIAAFGNDMVINFETD

3D structure

• PDB: 6lqr Cryo-EM structure of 90 S small ribosomal subunit precursors in transition states.
• Chain: RE
• Resolution: 8.6 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	RE	N171 N173 K175 E215 K217 K306 P790 K791 W792 R858 R865	N72 N74 K76 E116 K118 K200 P669 K670 W671 R737 R744
BS02	peptide	RE	T1062 R1063 V1070 T1090 G1092 P1188	T915 R916 V923 T943 G945 P1030

Gene Ontology

Molecular Function

• GO:0003674 molecular_function
• GO:0003723 RNA binding
• GO:0005515 protein binding

Biological Process

• GO:0000028 ribosomal small subunit assembly
• GO:0006364 rRNA processing
• GO:0006409 tRNA export from nucleus
• GO:0030490 maturation of SSU-rRNA
• GO:0042254 ribosome biogenesis
• GO:0042790 nucleolar large rRNA transcription by RNA polymerase I
• GO:0060962 regulation of ribosomal protein gene transcription by RNA polymerase II

Cellular Component

• GO:0005634 nucleus
• GO:0005654 nucleoplasm
• GO:0005730 nucleolus
• GO:0030686 90S preribosome
• GO:0032040 small-subunit processome
• GO:0032545 CURI complex
• GO:0034456 UTP-C complex
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:6lqr, PDBe:6lqr, PDBj:6lqr
• PDBsum: 6lqr
• PubMed: 32943522
• UniProt: P53254|UTP22_YEAST U3 small nucleolar RNA-associated protein 22 (Gene Name=UTP22)