Structure of PDB 4lel Chain RE

Receptor sequence

>4lelRE (length=205) Species: 300852 (Thermus thermophilus HB8)

MKGILGVKVGMTRIFRDDRAVPVTVILAGPCPVVQRRTPEKDGYTAVQLG
FLPQNPKRVNRPLKGHFAKAGVEPVRILREIRDFNPEGDTVTVEIFKPGE
RVDVTGTSKGRGFAGVMKRWNFAGGPDSHGAHKIHRHPGSIGNRKTPGRV
YKGKKMAGHYGAERVTVMNLEVVDVIPEENLLLVKGAVPGPNGGLVIVRE
TKKAA

3D structure

• PDB: 4lel Structural insights into +1 frameshifting promoted by expanded or modification-deficient anticodon stem loops.
• Chain: RE
• Resolution: 3.9 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	RE	M11 T12 R13 Q35 K41 D42 Y44 R58 N60 R61 P62 K64 I77 L78 R79 E80 R82 S108 K109 G110 R111 G112 F113 G115 M117 K118 R119 N121 F122 A123 P126 D127 S128 H129 G130 A131 H132 K133 I134 H135 R136 H137 P138 G139 S140 I141 G142 N143 R144 K145 T146 G148 R149 V150 Y151 K152 K154 M156 A157 H159 R164 V165 T166 M168 K185 A187 P189 P191 N192	M11 T12 R13 Q35 K41 D42 Y44 R58 N60 R61 P62 K64 I77 L78 R79 E80 R82 S108 K109 G110 R111 G112 F113 G115 M117 K118 R119 N121 F122 A123 P126 D127 S128 H129 G130 A131 H132 K133 I134 H135 R136 H137 P138 G139 S140 I141 G142 N143 R144 K145 T146 G148 R149 V150 Y151 K152 K154 M156 A157 H159 R164 V165 T166 M168 K185 A187 P189 P191 N192

Gene Ontology

Molecular Function

• GO:0003735 structural constituent of ribosome
• GO:0019843 rRNA binding

Biological Process

• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:4lel, PDBe:4lel, PDBj:4lel
• PDBsum: 4lel
• PubMed: 25128388
• UniProt: Q5SHN8|RL3_THET8 Large ribosomal subunit protein uL3 (Gene Name=rplC)