Structure of PDB 5j3c Chain QY

Receptor sequence

>5j3cQY (length=259) Species: 331111 (Escherichia coli O139:H28 str. E24377A)

QVLLLPKDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRV
EIMSASEGEHGGYKEIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRI
HTSACTVAVMPELPDAELPDINPADLRIDTFRSSGAGGQHVNTTDSAIRI
THLPTGIVVECQDERSQHKNKAKALSVLGARIHAAEMAKRQQAEASTRRN
LLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVMEGKLDMLIEPIIQ
EHQADQLAA

3D structure

• PDB: 5j3c Uniformity of Peptide Release Is Maintained by Methylation of Release Factors.
• Chain: QY
• Resolution: 3.04 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	QY	R116 R228 S229 S230 G231 A232 G233 G234 Q235 H236 V237 N238 T239 T240 S242 V255 E256 C257 D259 R261 Q263 H264 R277 R294 R295	R20 R132 S133 S134 G135 A136 G137 G138 Q139 H140 V141 N142 T143 T144 S146 V159 E160 C161 D163 R165 Q167 H168 R181 R198 R199
BS02	rna	QY	G118 T119 G120 G121 R183 S192 Q193 G194 R195 D302 R316	G22 T23 G24 G25 R87 S96 Q97 G98 R99 D206 R220
BS03	rna	QY	G233 G234 Q235 H236 E260 R261	G137 G138 Q139 H140 E164 R165
BS04	rna	QY	G120 E123 Q185 P188 T190 Q193 R195 I196 H197 T198	G24 E27 Q89 P92 T94 Q97 R99 I100 H101 T102

Gene Ontology

Molecular Function

• GO:0003747 translation release factor activity
• GO:0005515 protein binding
• GO:0016149 translation release factor activity, codon specific
• GO:0043022 ribosome binding

Biological Process

• GO:0006412 translation
• GO:0006415 translational termination

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:5j3c, PDBe:5j3c, PDBj:5j3c
• PDBsum: 5j3c
• PubMed: 27681416
• UniProt: P0A7I0|RF1_ECOLI Peptide chain release factor RF1 (Gene Name=prfA)